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目的 :观察 5 -HT1A受体激动剂乌拉地尔 (urapidil)对小鼠吗啡戒断反应、血浆及脑内NO含量的影响。方法 :皮下注射定量吗啡 ,建立小鼠吗啡依赖模型。第 6天早 8:0 0用不同剂量的 5 -HT1A受体激动剂乌拉地尔给小鼠腹腔注射 (ip) ,8:2 0纳络酮催瘾 ,观察小鼠戒断时出现跳跃反应次数和体重丢失 ,评定小鼠戒断反应的强度。用硝酸还原法检测血浆和脑内NO的含量。结果 :三种不同剂量 (10 0 ,2 0 0 ,40 0mg kg-1)的乌拉地尔可抑制小鼠吗啡戒断反应 ,并在此剂量范围内呈量效关系。其中 40 0mg kg-1的乌拉地尔可使血中NO升高 ,但使脑内NO含量降低。结论 :5 -HT1A受体激动剂乌拉地尔可抑制小鼠吗啡戒断反应及脑内NO含量的升高
OBJECTIVE: To observe the effects of 5-HT1A receptor agonist urapidil on morphine withdrawal response, plasma and brain NO content in mice. Methods: Quantitative morphine was injected subcutaneously to establish a mouse model of morphine dependence. On day 6, 8: 0 0, mice were injected intraperitoneally (ip) with different doses of 5-HT1A receptor agonist urapidil and 8: 20 naloxone. The mice were observed jumping reaction Frequency and weight loss, assessed the intensity of withdrawal response in mice. Nitric acid reduction method was used to detect the content of NO in plasma and brain. Results: Urapidil at three different dosages (10 0, 200, 40 0 mg kg -1) could inhibit the morphine withdrawal response in mice and showed a dose-response relationship within this dose range. Among them, 400 mg of Urapidil could increase NO in blood, but decreased NO in brain. Conclusion: Urapidil, a 5-HT1A receptor agonist, can inhibit the morphine withdrawal response and NO content in brain of mice