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探讨建立一种脊髓灰质炎病毒Sabin株灭活疫苗(IPV)D抗原准确定量的方法,并尝试用该方法取代微量中和实验,为人群脊灰抗体的监测建立一种更快速、简便的替代方法.应用抗3个型Sabin株D抗原的单克隆抗体的ELISA法与本室建立的多抗方法进行比较,同时对Sabin IPV中3个型D抗原含量进行测定,并用单克隆抗体法与多抗方法及中和实验同时对80份人血清进行抗脊灰抗体的检测.经统计学处理发现单克隆抗体法与多抗方法测定D抗原含量没有显著性差异,单克隆抗体间接ELISA法与多抗方法及微量中和实验3种方法对血清脊灰抗体检测也没有显著性差异.结果表明可用单克隆抗体间接ELISA法或多抗方法对IPV的D抗原进行准确定量,并可用单克隆抗体间接ELISA法或多抗方法取代微量中和实验对人群血清脊灰抗体进行检测.
To establish a method for accurate quantification of D antigen of Sabin inactivated vaccine (IPV) of poliovirus and try to use this method instead of micro-neutralization experiment to establish a more rapid and simple alternative for the monitoring of poliovirus in the population Methods The monoclonal antibody against Sabin strain D of 3 Sabin strains was compared with that of the polyclonal antibody against Sabin IPV by ELISA method. The levels of 3 D antigen in Sabin IPV were assayed by ELISA. Anti-method and neutralization test at the same time on 80 human serum anti-polio antibody detection.Statistical analysis found that monoclonal antibody and polyclonal anti-D antibody content was no significant difference, indirect ELISA and monoclonal antibody Anti-method and micro-neutralization test of the three methods of serological detection of antibodies to polio also showed no significant difference.The results show that monoclonal antibody can be indirect ELISA or polyclonal anti-IPV D antigen can be accurately quantified and monoclonal antibody can be used indirectly ELISA or multi-antibody method instead of micro-neutralization test to detect serum serogroup antibodies.