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在人淋巴细胞和原核细胞上比较了１０－羟基喜树碱（ＨＣＴ）的诱变性。用松胞素阻断的双核淋巴细胞（ＢＮＬ）来观察微核的形成．检测了浓度为０．６２５，０．１２５，０．２５和０．５０μｇ／ｍＬ的ＨＣＴ诱发ＢＮＬ的微核率．所有剂量组含微核的ＢＮＬ均明显增加并呈现剂量关系．相反ＨＣＴ浓度达３００μｇ／皿时并不诱发ＳａｌｍｏｎｅｌｌａｔｙｐｈｉｍｕｒｉｕｍＴＡ９７．ＴＡ９８，ＴＡ１００，ＴＡ１０２的菌落回变数增加．ＨＣＴ浓度为１０．２０μｇ／ｍｌ并不明显改变小牛胸腺ＤＮＡ的圆二色谱的形。这一差别可能是由於ＨＣＴ不直接作用放ＤＮＡ而涉及ＤＮＡ拓扑酶．
The mutagenicity of 10-hydroxycamptothecin (HCT) was compared on human lymphocytes and prokaryotes. The formation of micronuclei was observed with cytokine-blocked binucleated lymphocytes (BNLs). The micronucleus rates of BNT induced by HCT at concentrations of 0.625, 0.125, 0.25 and 0.50 μg / mL were examined. All doses of micronuclei containing BNL were significantly increased and dose-related. On the contrary HCT concentration of 300μg / dish does not induce Salmonella typhimuriumTA97. TA98, TA100, TA102 colony return variable increased. The HCT concentration of 10.20 μg / ml did not significantly alter the circular dichroism of calf thymus DNA. This difference may be due to the fact that HCT does not act directly on DNA but involves DNA topoisomerases.