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目的检测肝素结合表皮生长因子(HB-EGF)及其受体(EGFR)在窒息新生大鼠肠黏膜组织中的表达,并探讨其在窒息新生大鼠肠道损伤修复过程中的意义。方法将100只健康Wistar新生大鼠随机分成窒息复氧后3、6、12、24、48h组及其相应对照组,每组10只。建立常压窒息模型,应用免疫组化SP法检测肠黏膜组织HB-EGF和EGFR的表达,使用Biosens数字影像系统测定其阳性区平均积分吸光度值(A)。并进行HE染色,观察肠黏膜组织形态学及肠黏膜损伤指数(IMDI)的变化。结果窒息组肠黏膜组织在窒息复氧后3h即出现损伤,以复氧后24 h最为显著,之后开始恢复。HB-EGF表达在窒息复氧后呈一过性增高,复氧后12h达高峰(85.96±4.82),之后开始下降,与同时间点对照组相比差异均有统计学意义(P<0.05)。HB-EGF的表达与IMDI呈显著正相关(r=0.549,P<0.01)。EGFR的表达在复氧后24h达高峰(104.81±6.69),之后开始下降,与同时间点对照组相比差异有统计学意义(P<0.05)。EGFR的表达与IMDI呈显著正相关(r=0.627,P<0.01)。结论HB-EGF和EGFR可能在窒息复氧后新生鼠肠黏膜损伤早期修复过程中发挥重要作用。
Objective To detect the expression of heparin-binding epidermal growth factor (EGF-EGF) and its receptor (EGFR) in intestinal mucosa of neonatal rats with asphyxia and to investigate the significance of the expression of HB-EGF and its receptor in the repair of intestinal injury induced by asphyxia in neonatal rats. Methods 100 healthy Wistar newborn rats were randomly divided into 3, 6, 12, 24 and 48 hours after reoxygenation as well as their corresponding control groups, with 10 in each group. The model of normal pressure asphyxia was established. The expression of HB-EGF and EGFR in intestinal mucosa was detected by immunohistochemical SP method. The mean integral absorbance (A) of positive area was measured by Biosens digital imaging system. The changes of intestinal mucosal histology and intestinal mucosal injury index (IMDI) were observed by HE staining. Results Asphyxia group mucosa tissue 3h after asphyxial reoxygenation injury, reoxygenation 24 h after the most significant, and then began to recover. The expression of HB-EGF was transiently increased after asphyxia and peaked at 12h after reoxygenation (85.96 ± 4.82), and then began to decline. Compared with the control group at the same time point, the difference was statistically significant (P <0.05) . HB-EGF expression was positively correlated with IMDI (r = 0.549, P <0.01). The expression of EGFR peaked at 24h after reoxygenation (104.81 ± 6.69), then began to decline, which was significantly different from that of the control group at the same time (P <0.05). The expression of EGFR was positively correlated with IMDI (r = 0.627, P <0.01). Conclusion HB-EGF and EGFR may play an important role in the early repair of intestinal mucosal injury after asphyxia and reoxygenation.