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使用同源重组方法构建mex S基因突变菌株PAO1mex S::Ω,检测其与铜绿假单胞菌野生型菌株PAO1泳动,蹭行运动及蜂群运动能力.结果表明:与野生型菌株相比,PAO1mex S::Ω这3种运动能力均显著增加;构建mex S互补菌株PAO1mex S::ΩC,通过检测互补株的泳动、蹭行运动及蜂群运动能力,发现mex S基因回复了突变株这3种运动能力.为了探索mex S基因调控这3种运动能力的分子机制,进一步提取了PAO1与PAO1mex S::Ω菌株RNA,使用RNA测序技术比较两者全基因组转录水平,结果发现与野生型菌株相比,PAO1mex S::Ω中部分运动能力相关基因的表达水平发生了明显地变化.本文首次报道mex S基因影响铜绿假单胞菌的运动能力.
The homologous recombination method was used to construct the mutant strain PAO1mex S :: Ω of mex S gene, and its ability of swimming and rubbing against P. aeruginosa PAO1 was detected. The results showed that compared with the wild-type strain , PAO1mex S :: Ω all increased significantly. The mex S gene was found to be complementary to PAO1mex S :: ΩC by detecting the motility, reciprocity and colony motility of the complementary strains. Strain in order to explore the molecular mechanism of mex S gene regulation of these three kinds of exercise ability to further extract PAO1 and PAO1mex S :: Ω strain RNA RNA sequencing comparison of the two genome-wide transcription levels and found that Compared with the wild-type strain, the expression level of some exercise-related genes in PAO1mex S :: Ω changed obviously.In this paper, we report for the first time that the mexS gene affects the exercise ability of Pseudomonas aeruginosa.