论文部分内容阅读
Background Icariine is a flavonoid isolated from a traditional Chinese medicine Epimedium pubescens and is the mainactive compound of it.Recently,Epimedium pubescens was found to have a therapeutic effect on osteoporosis.But themechanism is unclear.The aim of the study was to research the effect of Icariine on the proliferation and differentiation ofhuman osteoblasts.Methods Human osteoblasts were obtained by inducing human marrow mesenchymal stem cells(hMSCs)directionallyand were cultured in the presence of various concentrations of Icariine.3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT)test was used to observe the effect of Icariine on cell proliferation.The activity ofalkaline phosphatase(ALP)and the amount of calcified nodules were assayed to observe the effect on cell differentiation.The expression of bone morphogenetic protein 2(BMP-2)mRNA was detected by reverse transcriptase-polymerasechain reaction(RT-PCR).Results Icariine(20μg/ml)increased significantly the proliferation of human osteoblasts.And,Icariine(10μg/ml and 20μg/ml)increased the activity of ALP and the amount of calcified nodules of human osteoblasts significantly(P<0.05).BMP-2 mRNA synthesis was elevated significantly in response to Icariine(20μg/ml).Conclusions Icariine has a direct stimulatory effect on the proliferation and differentiation of cultured human osteoblastcells in vitro,which may be mediated by increasing production of BMP-2 in osteoblasts.
Background Icariine is a flavonoid isolated from a traditional Chinese medicine Epimedium pubescens and is the mainactive compound of it.Recently,Epimedium pubescens was found to have a therapeutic effect on osteoporosis.But themechanism is unclear.The aim of the study was to research the effect Of Icariine on the proliferation and differentiation ofhuman osteoblasts.Methods Human osteoblasts were obtained by inducing human marrow mesenchymal stem cells(hMSCs)directionallyand were cultured in the presence of various concentrations of Icariine.3-(4,5-dimethylthiazol-2-yl) -2,5-diphenyltetrazolium bromide(MTT)test was used to observe the effect of Icariine on cell proliferation.The activity ofalkaline phosphatase(ALP) and the amount of calcified nodules were assayed to observe the effect on cell differentiation.The expression of bone Morphogenetic protein 2 (BMP-2) mRNA was detected by reverse transcriptase-polymerase chain reaction (RT-PCR). Results Icariine (20μg/ml) increased significantly th e proliferation of human osteoblasts. And, Icariine (10 μg/ml and 20 μg/ml) increased the activity of ALP and the amount of calcified nodules of human osteoblasts significantly (P<0.05). BMP-2 mRNA synthesis was elevated significantly in response to Icariine(20μg/ml).Conclusions Icariine has a direct stimulatory effect on the proliferation and differentiation of cultured human osteoblastcells in vitro,which may be mediated by increasing production of BMP-2 in osteoblasts.