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本研究比较了生长调节剂、ＡｇＮＯ３、活性炭、碳源、基本培养基、光照度、胚发育时期以及基因型等对龙眼幼胚培养诱导愈伤组织的影响．从幼胚培养中，诱导出多种类型的愈伤组织，经继代培养并筛选，获得松散、生长旺盛、胚胎发生能力极强的松散型胚性愈伤组织．该类型胚性愈伤组织适宜建立悬浮细胞系和分离原生质体，并且转移至含体积分数为０．０５椰子汁的ＭＳ固体培养基上，形成大量的胚状体．采用在附加１ｍｇＬ－１２，４－Ｄ的ＭＳ培养基与附加１ｍｇＬ－１２，４－Ｄ、０．５ｍｇＬ－１ＫＴ和５ｍｇＬ－１ＡｇＮＯ３的ＭＳ培养基交替继代培养，该类型胚性愈伤组织可长期保持
This study compared the effects of growth regulator, AgNO3, activated carbon, carbon source, basal medium, light intensity, embryonic developmental period and genotype on the induction of callus from longan immature embryos. From immature embryo culture, many types of callus were induced, and after subculture and selection, loose embryogenic callus with loose embryogenesis and strong embryogenesis ability was obtained. This type of embryogenic callus is suitable for establishing a suspension cell line and isolating protoplasts and is transferred to MS solid medium containing 0.05 coconut juice volume fraction to form a large number of embryoid bodies. The subcultures were subcultured with MS medium supplemented with 1 mg L-12,4-D and MS medium supplemented with 1 mg L-12,4-D, 0.5 mg L-1 KT and 5 mg L-1 AgNO3, Long-term maintenance