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用分子沉积法，在多孔三甲胺基聚苯乙烯载体上成功地固定化了双层葡萄糖异构酶。结果表明，这种新固定化酶方法能使单位重量的固定化酶活力及蛋白载量成倍增加，活力达到１２００ＩＵ／ｇ湿胶。与吸附法相比，酶活力提高１倍；其半衰期与吸附法相比则基本相同，为４５ｄ．还确定了最佳固定化条件并测定了固定化酶的性质。固定化酶的最适反应温度比液相酶提高１５℃，而最适ｐＨ值则没有改变。Ｋ＿ｍ值与液相酶的相比有一定程度的增加，稳定性与吸附法相比则基本相同。
Double-layer glucose isomerase was successfully immobilized on porous trimethylammonium polystyrene support by molecular deposition. The results showed that the new immobilized enzyme method can double the activity of immobilized enzyme per unit weight and the protein load, and the activity reached 1200 IU / g wet glue. Compared with the adsorption method, the enzyme activity increased by 1 time; its half-life compared with the adsorption method is basically the same, for 45d. The best immobilization conditions were also determined and the properties of immobilized enzymes were determined. The optimum reaction temperature of immobilized enzyme was 15 ℃ higher than that of liquid phase enzyme, while the optimum pH value did not change. K_m value compared with the liquid phase enzyme to a certain extent, stability and adsorption method is basically the same.