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Objective: To evaluate the effect of an important dietary supplement on sperm quantity and quality breeding rams of the three main breeds of sheep at the artificial insemination center, called Belhandjir, in the region of Naama in Algeria. Methods: Twenty-four breeding rams of the three major breeds in Algeria, namely Ouled Djellal, Hamra and Rumbi breeds are divided into two groups CR and SR, and subjected to two different diets. The first group CR followed a regime R based on barley and fodder and the second SR group to follow a regime R + a vitamin and mineral dietary supplement for 15 weeks of collection. Analysis of sperm quality (motility) and quantity (volume, concentration and doses produced) were followed after collection. Results: The quantitative effect was more significant in supplemented rams SR, volume and concentration were higher in rams SR. Therefore, the doses produced differ significantly compared to the group of control rams CR. Results of motility were more mixed, motility showed a significant difference in rams supplemented of Ouled Djellal breed only; however, the other two breeds Hamra and Rumbi, showed no significant differences. Conclusions: Supplementation could significantly improve the characteristics of quantitative sperm in the breeding rams of the three main Algerian sheep breeds. Motility, only qualitative parameter studied, was not significantly improved.