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本文采用0.2%甲烷磺酸乙酯(EMS)溶液(pH7)处理大豆稳定系LF81-9903和F_1(合交77-153×哈80-3249)种子,研究大豆油主要脂肪酸的变异,以探求改善大豆油品质的有效技术方法。试验表明,经EMS处理后,大豆油中脂肪酸含量发生遗传改变,其方差明显提高,较未处理对照提高1.2~6倍。广义遗传力、遗传进度发生一定程度的变化。出现一些亚麻酸含量明显下降、亚油酸含量降低较少甚至增加的优良突变体,如518-1,643-3等。现正进行抗病性、丰产性的培育。0.2%EMS对诱发大豆脂肪酸有益突变是有效的。
In this paper, the seeds of soybean stable lines LF81-9903 and F_1 (crosses 77-153 × ha 80-3249) were treated with 0.2% methanesulfonic acid ethyl ester (EMS) solution (pH7) to study the variation of major fatty acids in soybean oil, Effective technical method of soybean oil quality. The results showed that after EMS treatment, the fatty acid content of soybean oil changed genetically, and the variance was significantly increased, which was 1.2 to 6 times higher than that of the untreated control. Generalized heritability, genetic progress to some degree of change. Some linolenic acid content was significantly decreased, linoleic acid content decreased less or even increased good mutants, such as 518-1,643-3 and so on. Currently carrying out disease resistance, high yielding cultivation. 0.2% EMS is effective in inducing beneficial mutations in soybean fatty acids.