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目的研究益生菌及肠道菌群对低聚甘露糖的降解情况。方法(1)将5名志愿者粪便样品,13株纯菌以及7种微生态制剂接种到VI-MO液体培养基中,37℃厌氧培养24或72h,取样并采用TLC(薄层层析)检测降解情况;(2)选取7种微生态制剂中降解能力较强的乐塞益生菌胶囊(LS)中的益生菌作为筛选源,采用VI-MO固体培养基分离纯化低聚甘露糖的单一降解菌;(3)将B.uniformis L8和L.plantarum LS1A同时接种到VI-MO液体培养基中,37℃厌氧培养72h,取样并采用TLC检测降解情况。结果 (1)不同人体肠道菌群和微生态制剂对低聚甘露糖的利用情况存在较大差异,B.uniformis L8和B.xylanisolvens C5可利用大聚合度低聚甘露糖;(2)从微生态制剂中分离得到的L.plantarum LS1A可利用小聚合度低聚甘露糖;(3)B.uniformis L8利用MO产生的小寡糖能被L.plantarum LS1A利用。结论低聚甘露糖被拟杆菌降解释放出的小聚合度寡糖可以被乳酸菌利用,作为益生元很有可能改善肠道菌群的结构。
Objective To study the degradation of oligo-mannose by probiotics and intestinal microflora. Methods (1) Five volunteers’ stool samples, 13 strains of pure bacteria and 7 kinds of probiotics were inoculated into VI-MO liquid medium and incubated anaerobically for 24 or 72 hours at 37 ° C. Samples were taken and analyzed by TLC ) To detect the degradation; (2) Select Probiotics from probiotics probiotic capsule (LS), which has strong biodegradability in seven kinds of probiotics, as the screening source, using VI-MO solid medium to separate and purify the oligo-mannose (3) B.uniformis L8 and L.plantarum LS1A were inoculated into VI-MO liquid medium at the same time, cultured anaerobically at 37 ° C for 72h, sampled and analyzed by TLC. Results (1) There was a great difference in the use of mannose between different human intestinal microflora and probiotics. Both B.uniformis L8 and B.xylanisolvens C5 could utilize the high degree of polymerization of mannose. (2) L.plantarum LS1A isolated from probiotics can utilize oligomeric mannose with a low degree of polymerization. (3) The oligosaccharides produced by B.uniformis L8 using MO can be utilized by L. plantarum LS1A. CONCLUSIONS Oligomeric oligosaccharides released by the degradation of oligo-mannose by Bacteroidetes can be utilized by lactic acid bacteria. As a prebiotics, it is possible to improve the structure of intestinal microflora.