论文部分内容阅读
构建编码乙型肝炎病毒（ＨＢＶ）表面蛋白Ｓ的重组质粒ｐＣＲ３．１－Ｓ。将之直接肌肉注射Ｂａｌｂ／ｃ小鼠，观察小鼠 ＨＢＶ特异的免疫应答。以 ＥＬＩＳＡ法检测小鼠血清，３Ｈ－ＴｄＲ掺入法测定淋巴细胞增殖，５１Ｃｒ ４ｈ释放法检测淋巴细胞杀 伤功能。结果表明，与空载体对照组相比较，基因疫苗诱发小鼠产生良好的抗ＨＢｓ反应及ＨＢＶ特异的细胞免疫应答 （Ｐ＜０．０５）。提示，基因疫苗ｐＣＲ３．１－Ｓ有可能成为控制ＨＢＶ感染的又一武器。
Recombinant plasmid pCR3.1-S encoding hepatitis B virus (HBV) surface protein S was constructed. Balb / c mice were injected intramuscularly directly to observe the specific HBV immune response in mice. Serum was detected by ELISA, lymphocyte proliferation was measured by 3H-TdR incorporation, and lymphocyte cytotoxicity was measured by 51Cr 4h release assay. The results showed that compared with the control group, the gene vaccine induced good anti-HBs response and HBV-specific cellular immune response (P <0.05). Tip, gene vaccine pCR3.1-S may become another weapon to control HBV infection.