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To study the mechanism of hepatitis C virus (HCV) replication and to test gene therapy for hepatitis C, a human liver cell line expressed HCV RNA polymerase has been established.Methods NS5B gene has been transfected into Huh 7 cells by lipofectamine The results of transfection were confirmed by PCR and Southern blot analysis, and the level of the non structural protein 5B (NS5B) in Huh 7 cells was detected by Western blot analysis Results There were NS5B gene fragments and the expression of NS5B protein in Huh 7c cells transfected with pTeT NS5B or pcDNA NS5B plasmid Conclusions We have established a HCV RNA polymerase expression system in Huh 7 cells which can be further used to analyze the mechanism of HCV replication and provide a cell model for gene therapy in vitro
To study the mechanism of hepatitis C virus (HCV) replication and to test gene therapy for hepatitis C, a human liver cell line expressed HCV RNA polymerase has been established. Methods NS5B gene has been transfected into Huh 7 cells by lipofectamine The results of transfection were confirmed by PCR and Southern blot analysis, and the level of the non structural protein 5B (NS5B) in Huh 7 cells was detected by Western blot analysis Results were were NS5B gene fragments and the expression of NS5B protein in Huh 7c cells transfected with pTeT NS5B or pcDNA NS5B plasmid Conclusions We have established a HCV RNA polymerase expression system in Huh 7 cells which can be further used to analyze the mechanism of HCV replication and provide a cell model for gene therapy in vitro