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目的探讨硫氧还原蛋白(Sulfiredoxin,Srx)与上皮细胞黏附分子E-钙黏蛋白(E-cadherin)在宫颈鳞癌组织中的表达及临床意义。方法采用免疫组织化学SABC法检测30例正常宫颈上皮组织(NC)、20例重度子宫颈上皮内瘤变(HSIL)及104例宫颈鳞状细胞癌(CSCC)组织中Srx和E-cadherin蛋白的表达情况,分析两者与宫颈鳞癌临床病理特征的关系及两者表达的相关性。结果 Srx在宫颈鳞癌组织中呈现高表达,而E-cadherin在鳞癌组织中表达明显降低,2蛋白阳性表达率在3组中比较差异具有统计学意义(P<0.05),且Srx与E-cadherin蛋白表达呈负相关。宫颈鳞癌组织中Srx的表达与淋巴结转移、肿瘤浸润深度及有无脉管浸润有关(P<0.05),而与患者年龄、临床分期、肿瘤大小及癌组织分化程度无关(P>0.05)。E-cadherin的阳性表达率与肿瘤大小、组织分化程度及淋巴结转移关系密切(P<0.05)。结论宫颈鳞癌组织中Srx的高表达与恶性临床病理特征密切相关,并与Ecadherin蛋白表达呈负相关,提示Srx可能通过调控EMT促进宫颈鳞癌进展。
Objective To investigate the expression of Sulfiredoxin (Srx) and epithelial cell adhesion molecule E-cadherin in cervical squamous cell carcinoma and its clinical significance. Methods The expressions of Srx and E-cadherin proteins in 30 cases of normal cervical epithelium (NC) The relationship between them and the clinicopathological characteristics of cervical squamous cell carcinoma and their correlations were analyzed. Results The expression of Srx in squamous cell carcinoma of the cervix was high, while the expression of E-cadherin in squamous cell carcinoma was significantly lower than that in the squamous cell carcinoma (P <0.05). The positive expression rate of Src protein was significantly higher in the three groups (P <0.05) -cadherin protein expression was negatively correlated. The expression of Srx in cervical squamous cell carcinoma was related to the lymph node metastasis, tumor invasion depth and the presence or absence of vascular invasion (P <0.05), but not to the age, clinical stage, tumor size and the degree of cancer differentiation (P> 0.05). The positive rate of E-cadherin was closely related to tumor size, histological grade and lymph node metastasis (P <0.05). Conclusion The high expression of Srx in cervical squamous cell carcinoma is closely related to the clinicopathological features of malignant and negatively correlated with the expression of Ecadherin protein, suggesting that Srx might promote the progression of cervical squamous cell carcinoma by regulating EMT.