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目的评价胶体金法在内蒙古布鲁氏菌病监测地区的现场应用效果。方法选择内蒙古布鲁氏菌病监测地区重点人群,采用虎红平板凝集试验(RBPT)、试管凝集试验(SAT)和胶体金法进行检测,并对血清学结果进行对比分析。结果共检测1 088份疑似布病患者血清,在RBPT、SAT检测均为阳性的645例中胶体金法检测为阳性的483例、阴性162例,吻合率为74.8%;在RBPT、SAT检测均为阴性或达不到布病诊断标准的443例中胶体金法检测为阳性的11例,假阳性率为2.5%。结论布鲁氏菌抗体检测试剂(胶体金法)检测布鲁氏菌病与RBPT和SAT检出的阳性布病患者的吻合率不高,该试剂的推广使用价值还需更多实验数据进一步支持。
Objective To evaluate the field application of colloidal gold method in brucellosis surveillance in Inner Mongolia. Methods The key population of Brucellosis surveillance in Inner Mongolia were selected and detected by RBPT, SAT and colloidal gold assay. The serological results were compared and analyzed. Results A total of 1 088 suspected brucellosis patients were detected. Among the 645 cases with positive RBPT and SAT tests, 483 cases were positive for colloidal gold assay and 162 cases were negative. The anastomosis rate was 74.8% In the 443 cases that were negative or did not reach the diagnostic criteria for brucellosis, 11 cases were positive by colloidal gold assay and the false positive rate was 2.5%. Conclusion Brucella antibody detection reagent (colloidal gold method) to detect brucellosis and RBPT and SAT detection of positive brucellosis patients is not high, the use of the reagent need further experimental data to further support the value .