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目的研究免疫接种次数对牛血清及乳汁中特异性抗体效价的影响。方法以9株人肠道病原菌制备的混合抗原对孕牛进行系统免疫,其中对照组免疫接种4次,实验组免疫接种6次,分娩后收集血清及乳汁,检测其中特异性抗体的效价。结果实验组和对照组分娩14d后血清及常乳中特异性抗体效价比较,差异有高度统计学意义(P﹤0.01):对照组血清效价平均为28～210,实验组血清效价平均为210～212。对照组乳汁效价平均为25～26,实验组乳汁效价平均为26～27,并且随着时间的推移差异越来越显著。结论多次免疫接种,能使牛血清中特异性抗体效价在长时间内保持较高水平,这也保证了免疫乳中特异性抗体效价在长时间内保持较高水平。
Objective To study the effect of immunization times on the titer of specific antibodies in bovine serum and milk. Methods The pregnant cows were immunized with mixed antigen prepared by 9 strains of human enteric pathogenic bacteria. The control group was immunized four times and the experimental group immunized six times. Serum and milk were collected after delivery to test the titer of specific antibodies. Results The titer of specific antibodies in serum and regular milk of experimental group and control group after 14 days of delivery was highly statistically significant (P <0.01). The mean serum titer of control group was 28-210. The mean serum titer of experimental group was For 210 ~ 212. The average milk titer in the control group was 25-26, and the average milk titer in the experimental group was 26-27, with the difference becoming more and more obvious over time. Conclusions Multiple vaccination can keep the titer of specific antibody in bovine serum to a high level for a long time, which ensures that the titer of specific antibody in immune milk will maintain a high level for a long time.