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目的:研究中药外敷对哮喘豚鼠板层小体及肺表面活性物质的影响。方法:将40只豚鼠随机分为正常组、中药外敷组、生理盐水组、地塞米松组;除正常组外,各组采用Hutson法造哮喘模型,治疗组用中药敷贴,生理盐水组予氯化钠溶液浸润纱布敷贴,地塞米松组予地塞米松磷酸钠注射液浸润纱布敷贴。2周后处死豚鼠,取肺组织采用透射电镜观察哮喘豚鼠肺泡的超微结构,高效液相色谱法测定各组豚鼠支气管肺泡灌洗液中磷脂成分的含量。结果:生理盐水组、地塞米松组豚鼠肺泡Ⅱ型上皮细胞内板层小体脱颗粒、空泡化严重,与中药外敷组比较有显著差异(P<0.01);中药外敷组Pc、Pe含量高于生理盐水组和地塞米松组(P<0.01);Pi含量高于正常组(P<0.01),但低于生理盐水组、地塞米松组(P<0.01);各组Ps含量无统计学差异(P>0.05)。结论:中药外敷能减轻哮喘豚鼠气道炎症,促进肺表面活性物质的磷脂分泌。
Objective: To study the effect of topical application of traditional Chinese medicine on lamina propria and pulmonary surfactant in asthmatic guinea pigs. Methods: Forty guinea pigs were randomly divided into normal group, external application group, normal saline group and dexamethasone group. Except for normal group, Hutson method was used to make asthma model in each group. The treatment group was treated with Chinese medicine, Sodium chloride solution impregnated gauze dressing, dexamethasone group dexamethasone sodium phosphate impregnated gauze dressing. The guinea pigs were sacrificed after 2 weeks, the ultrastructure of alveoli in asthmatic guinea pigs was observed by transmission electron microscopy in lungs, and the content of phospholipid in bronchoalveolar lavage fluid of guinea pigs in each group was determined by high performance liquid chromatography. Results: In the saline group and dexamethasone group, the laminae of alveolar type Ⅱ epithelial cells were degranulated and vacuolized seriously in the saline group and dexamethasone group (P <0.01). The contents of Pc and Pe in the external application group (P <0.01); Pi content was higher than that of normal saline group (P <0.01), but lower than that of saline group and dexamethasone group (P <0.01) Statistical difference (P> 0.05). Conclusion: The application of Chinese herbal medicine can relieve the airway inflammation of asthmatic guinea pigs and promote the phospholipid secretion of pulmonary surfactant.