蓖麻毒素对肝癌细胞核转录因子NF-κB的激活作用

• 作 者: 董巨莹 彭宣宪 药立波
• 机 构: 厦门大学生物系肿瘤细胞工程教育部重点实验室! 福建 厦门 361005,厦门大学生物系肿瘤细胞工程教育部重点实验室! 福建 厦门 361005,第四军医大学生化与分子生物学教研室! 陕西
• 出 处: 癌症
• 发表日期: 2000年12期
• 关键词: NF 核转录因子 肝癌 蓖麻毒素 肝癌细胞 激活作用 细胞毒性作用 细胞相互作用 配基 免疫细胞化学

目的：探讨蓖麻毒素细胞毒性作用的分子机理。方法：采用免疫细胞化学和 Western blot等方法观察蓖麻毒素作用于人肝癌细胞后，引起核转录因子 NFκ B的变化。结果： 100 pmol/L的蓖麻毒素处理细胞后，随着时间的延长（ 30 min、 1 h和 2 h）， NFκ B由胞浆逐渐向核内转移， 2 h后胞浆和胞核的着色均变浅，表明 NFκ B的活性略有减弱；随着蓖麻毒素浓度的增大，核内棕色颗粒也有加深的趋势。结论：本结果表明蓖麻毒素通过其糖配基与肝癌细胞相互作用后，能启动核转录因子 NFκ B的表达。 NFκ B激活在其抗癌作用中起着重要作用。

Objective: To investigate the molecular mechanism of ricin cytotoxicity. METHODS: Immunocytochemistry and Western blot were used to observe the effect of ricin on human hepatocellular carcinoma cells and induce the change of nuclear factor NFκB. RESULTS: After treatment with 100 pmol/L ricin, the NF  κ B was gradually transferred from the cytoplasm to the nucleus over time (30 min, 1 h, and 2 h). The cytoplasm and cytoplasm were detected 2 h later. The coloring of the nucleus became lighter, indicating that the activity of NF-κB was slightly decreased. With the increase of the ricin concentration, the brown granules in the nucleus also had a tendency to deepen. Conclusion: This result shows that ricin can initiate the expression of nuclear transcription factor NF  κ B through its interaction with hepatoma cells. Activation of NF-κB plays an important role in its anticancer effect.