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目的:研究胃癌组织中增殖细胞核抗原(PCNA)和细胞色素C(CytC)蛋白的表达、相互关系及其临床意义。方法:应用免疫组织化学S-P法,检测59例胃癌组织及10例正常胃黏膜组织中PCNA和CytC蛋白的表达。结果:10例正常胃黏膜组织中,PCNA和CytC蛋白表达率分别为20.0%(2/10)和10.0%(1/9),胃癌组织中PCNA和CytC蛋白的表达率分别为86.4%和78.0%,胃癌组织表达率高于正常胃黏膜组织(P<0.05或P<0.01)。在不同年龄、不同肿瘤大小的胃癌患者中PCNA和CytC的表达率比较差异无显著性(P>0.05),低分化患者PCNA的表达率高于高分化患者(P<0.05),低分化和中分化患者CytC蛋白表达率均低于高分化患者(P<0.01),胃癌组织中PCNA蛋白与CytC蛋白的表达频数差异无显著性(P>0.05)。结论:PCNA蛋白的高表达及CytC的低表达与胃癌的发生发展有关,提示二者在胃癌的发生和发展过程中分别通过不同途径来发挥生物学作用。
OBJECTIVE: To study the expression of PCNA and CytC protein in gastric carcinoma and its relationship with clinical significance. Methods: The expressions of PCNA and CytC in 59 cases of gastric carcinoma and 10 cases of normal gastric mucosa were detected by immunohistochemical S-P method. Results: The expression rates of PCNA and CytC in 20 normal gastric mucosa tissues were 20.0% (2/10) and 10.0% (1/9) respectively. The expressions of PCNA and CytC in gastric cancer tissues were 86.4% and 78.0% %, The expression rate of gastric cancer was higher than that of normal gastric mucosa (P <0.05 or P <0.01). There was no significant difference in the expression rates of PCNA and CytC between patients with different ages and different tumor sizes (P> 0.05). The expression of PCNA in poorly differentiated patients was higher than that in well-differentiated patients (P <0.05) The expression of CytC in differentiated patients was lower than that in well-differentiated patients (P <0.01). There was no significant difference in the expression frequency of CytC and PCNA in gastric cancer (P> 0.05). Conclusion: The high expression of PCNA and the low expression of CytC are associated with the occurrence and development of gastric cancer, suggesting that both of them play biological roles in different pathways during the development and progression of gastric cancer.