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用CO_2激光器的最佳剂量对来杭鸡种蛋进行照射并孵化。对孵化后的48天雏鸡、160天育成鸡、251天产蛋鸡分别剖杀取脾,将新鲜脾脏制成匀浆、冻融,用冻融后的匀浆液进行透析制成转移因子(TF),-30℃保存。
The best dose of CO2 laser was used to irradiate and hatch eggs of Lai Hang chicken. The hatched 48-day-old chickens, 160-day-old chickens and 251-day-old laying hens were dissected to remove the spleen, and the fresh spleen was homogenized, freeze-thawed, and dialyzed with the homogenate after thawing ), - 30 ℃ preservation.