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本研究评价黄原胶(xanthan gum,XG)对前交叉韧带切断术(anterior cruciate ligament transaction,ACLT)所致的兔膝关节骨关节炎(osteoarthritis,OA)模型中软骨损伤的保护作用,并探讨XG对OA软骨中caspase-3、Bax蛋白表达的影响。将60只雄性新西兰大耳白兔按随机数字表法分为6组,每组10只,随机选取1组为正常对照组(control组),剩余5组右膝采用ACLT建立OA模型,并根据药物干预不同分为模型组(model组)、XG-0.6 mg·k~(g-1)、XG-1.2 mg·k~(g-1)、XG-2.4 mg·k~(g-1)治疗组和玻璃酸钠(sodium hyaluronate,SH-1.2 mg·k~(g-1))治疗组。测量治疗过程中各组兔膝关节局部温度和膝关节宽度;治疗结束后,对各组兔关节进行大体形态学观察;通过HE染色对各组兔关节软骨组织病理学形态进行观察;Western blot法检测各组家兔软骨细胞中Bax和caspase-3的激活态cleaved caspase-3含量。实验结果表明,XG可抑制OA所致的膝关节局部温度的上升和膝关节宽度的增加,且具有剂量依赖性;XG具有改善由OA所致的股骨髁和胫骨平台形态学异常和组织损伤的作用;Western blot结果表明:与control组相比,model组和XG-0.6 mg·k~(g-1)组家兔膝关节软骨细胞中Bax和cleaved caspase-3水平显著增加(P<0.01),model组和XG-0.6 mg·k~(g-1)组间兔膝关节软骨细胞中Bax和cleaved caspase-3水平无显著性差别(P>0.05),且明显高于XG-1.2 mg·k~(g-1)和XG-2.4 mg·k~(g-1)组(P<0.01),XG-2.4 mg·k~(g-1)和XG-1.2 mg·k~(g-1)组间兔骨关节软骨细胞中cleaved caspase-3水平无显著性差别(P>0.05),XG-2.4 mg·k~(g-1)组兔骨关节软骨细胞中Bax水平低于XG-1.2 mg·k~(g-1)组(P<0.05)。综上所述,XG能够有效地保护OA中的软骨损伤,并能够抑制OA软骨中Bax和caspase-3蛋白的表达。
This study evaluated the protective effect of xanthan gum (XG) on cartilage injury in rabbit knee osteoarthritis (OA) model induced by anterior cruciate ligament transaction (ACLT) Effect of XG on the Expression of Caspase-3 and Bax in OA Cartilage. Sixty male New Zealand white rabbits were randomly divided into 6 groups according to random number table. Each group consisted of 10 rabbits. One group was selected randomly as control group. The other 5 groups were treated with ACLT to establish OA model. The drug intervention was divided into model group, XG-0.6 mg · kg -1, XG-1.2 mg · kg -1, XG-2.4 mg · k g -1, Treatment group and sodium hyaluronate (SH-1.2 mg · k ~ (g-1)) treatment group. The local knee joint temperature and knee width of each group were measured during treatment. Gross morphology of each group was observed after treatment. Histopathological changes of articular cartilage were observed by HE staining. Western blot The cleaved caspase-3 levels of Bax and caspase-3 in rabbit chondrocytes were detected. The experimental results show that XG can inhibit the increase of local temperature and knee width of knee joint induced by OA and has a dose-dependent manner. XG can improve morphological and tissue damage of the femoral condyle and tibial plateau caused by OA Western blot results showed that the levels of Bax and cleaved caspase-3 in articular chondrocytes of model group and XG-0.6 mg · kg -1 group were significantly increased compared with control group (P <0.01) , there was no significant difference in the levels of Bax and cleaved caspase-3 in rabbit knee articular chondrocytes between model group and XG-0.6 mg · k-g-1 group (P> 0.05), which was significantly higher than that of XG-1.2 mg · XG-2.4 mg · kg-1 and XG-1.2 mg · kg-1 g-1 and XG-2.4 mg · g-1 g- There was no significant difference in the cleaved caspase-3 level between the two groups (P> 0.05). The level of Bax in the osteoarticular chondrocytes of XG-2.4 mg · kg -1 group was lower than that of XG- 1.2 mg · k ~ (g-1) group (P <0.05). In summary, XG can effectively protect the cartilage injury in OA, and can inhibit the expression of Bax and caspase-3 protein in OA cartilage.