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目的检测系统性红斑狼疮(SLE)患者外周血CD4~+CD25~+FOXP3~+调节性T细胞(Treg)的百分比,并分析其与疾病活动的相关性。方法采用流式细胞仪检测28例SLE患者(其中活动组18例)及22名正常对照组的外周血CD4~+CD25~+FOXP3~+Treg的百分比,同时评估SLE疾病活动指数(SLEDAI)及检测血抗dsDNA水平,分析相关性。结果SLE患者外周血CD4~+CD25~+FOXP3~+Treg占CD4~+ T细胞的百分比较对照组降低(P<0.05),以活动组尤为明显,CD4~+CD25~+FOXP3~+Treg的百分比与SLEDAI呈明显的负相关(P<0.01),同时显示血抗dsDNA阳性组较阴性组患者外周血CD4~+CD25~+FOXP3~+Treg的百分比显著下降(P<0.01)。结论SLE患者外周血CD4~+CD25~+FOXP3~+Treg的百分比在活动期下降为明显,其在SLE的发病机制中可能起到重要的作用。
Objective To detect the percentage of CD4 ~ + CD25 ~ + FOXP3 ~ + regulatory T cells (Tregs) in peripheral blood of patients with systemic lupus erythematosus (SLE) and analyze its relationship with disease activity. Methods The percentages of CD4 ~ + CD25 ~ + FOXP3 ~ + Treg in peripheral blood of 28 SLE patients (18 in active group) and 22 normal controls were detected by flow cytometry. The SLE disease activity index (SLEDAI) and Blood anti-dsDNA levels were tested for correlation. Results The percentage of CD4 ~ + T cells in CD4 + CD25 ~ + FOXP3 + Tregs in peripheral blood of SLE patients was significantly lower than that in control group (P <0.05) (P <0.01). The percentage of CD4 ~ + CD25 ~ + FOXP3 ~ + Tregs in peripheral blood of patients with anti-dsDNA positive was significantly lower than that of negative patients (P <0.01). Conclusion The percentage of CD4 ~ + CD25 ~ + FOXP3 ~ + Treg in peripheral blood of SLE patients decreased significantly during the active phase, which may play an important role in the pathogenesis of SLE.