论文部分内容阅读
采用高效液相色谱技术,研究了不同发育时期宁杞1号和宁杞5号枸杞(Lycium barbarum)果皮和种子内源激素(玉米素(ZT)、赤霉素(GA3)、生长素(IAA)和脱落酶(ABA))含量与果实生长发育的关系。结果表明,宁杞5号果实横纵径和单粒重均大于宁杞1号,果实发育前期,尤其是缓慢生长期,是宁杞5号果实大小和重量积累的关键时期。宁杞1号种子中的生长素含量与果实横径和果实单粒重均呈极显著正相关,与果实纵径呈显著正相关。宁杞5号果皮中玉米素含量与果实横径和单粒重均呈显著正相关,种子中生长素含量与果实横径和单粒重均呈显著正相关。玉米素促进细胞的分裂,而细胞数目比细胞体积对决定果实大小的作用更大;在缓慢生长期(开花后8–25天),宁杞5号果皮和种子中的脱落酸含量均显著小于宁杞1号。以上两点可能是宁杞5号的横纵径和单粒重均大于宁杞1号的主要原因。宁杞1号果皮中的GA3与半纤维素酶(Cx)活性的变化相反,说明宁杞1号果实发育前期和中期果皮中高含量的GA3对细胞壁中Cx活性有一定的抑制作用,从而表现出果实膨大。宁杞5号果皮中的IAA与多聚半乳糖醛酸酶(PG)和果胶酯酶(PE),ZT与PE,ABA与Cx,种子中的ZT与PE的变化均相反,说明宁杞5号果实发育前期和中期果皮中高含量的IAA、ZT、ABA及种子中的ZT可促进果实的膨大。推测这可能是宁杞5号果实单粒重大于宁杞1号的主要原因之一。宁杞5号果皮中的ZT和种子中的IAA可以增强Cx的活性;宁杞1号果皮中的ABA可以增强PE的活性,进而促进果实的成熟。
High performance liquid chromatography (HPLC) was used to study the effects of endogenous hormones (zein (ZT), gibberellin (GA3), auxin (IAA) on the pericarp and seeds of Lycium barbarum at different developmental stages ) And shedding enzyme (ABA)) content and fruit growth and development. The results showed that the fruit length and single grain weight of Ningqi 5 were higher than that of Ningqi 1, and the early fruit development, especially the slow growth stage, was the key period for the fruit size and weight accumulation in Ningqi 5. The content of auxin in Ningqi 1 seed had extremely significant positive correlation with the fruit diameter and fruit single grain weight, and had a significant positive correlation with the fruit longitudinal diameter. The content of zein in Ningqi No.5 peel had a significant positive correlation with the fruit diameter and single grain weight. The content of auxin in seed was positively correlated with the fruit diameter and single grain weight. Zeatin promoted the cell division, and the number of cells was more important than the cell volume to determine the fruit size. In the slow growth stage (8-25 days after flowering), the abscisic acid content in the peel and seed of Ningqi 5 was significantly less than Ning Qi No. 1. The above two points may be the main reason for the Ningqiang No. 5 horizontal and vertical weight and grain weight are greater than Ningqi 1. The changes of GA3 and hemicellulase (Cx) activities in the pericarp of Ningqi 1 were opposite, indicating that high content of GA3 in the pericarp of Ningqiyi No.1 had a certain inhibitory effect on Cx activity in the cell wall, Fruit enlargement. The changes of IAA, PG and pectin esterase (PE), ZT and PE, ABA and Cx in peel of Ningqi No.5 and ZT and PE in seeds were opposite, indicating that Ningqi The high contents of IAA, ZT and ABA in the peel and the ZT in seeds during the early and middle stages of fruit development on the 5th could promote the fruit enlargement. It is speculated that this may be one of the main reasons why Ningqi 5 is larger than Ningqi 1 in grain weight. ZT in Ningqi No.5 and IAA in seeds can enhance the activity of Cx. ABA in Ningqi No.1 peel can enhance the activity of PE and further promote the fruit ripening.