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目的 研究降钙素基因相关肽(calcitonin gene-related peptide,CGRP)转基因对移植静脉病的防治作用,并探索作用机制.方法 将25只雄性新西兰白兔随机分为3组:实验组(n=8),取兔颈静脉,转染含CGRP基因的重组腺相关病毒2/1型载体(mosaic adeno-associated virus vector ripe 2/1 containing CGRP gene,AAV2/1-CGRP);载体组(n=9),转染含β-半乳糖苷酶基因z的重组腺相关病毒2/1型载体(mosaic adeno-associated virus vector tipe 2/1 containing LacZ gene,AAV2/1-LacZ)和对照组(n=8),采用生理盐水,反向端-侧吻合植入同侧颈动脉.于术后4周取标本行病理、CD68免疫组化、β-半乳糖苷酶原位染色.逆转录聚合酶链式反应(reverse transcription-polymerase chain reaction,RT-PCR)观察CGRP基因表达,实时荧光定量聚合酶链式反应(real-time polymerase chain reaction,real-time PCR)测定单核细胞趋化因子-1 (monocyte chemoattractant protein-1,MCP-1)、肿瘤坏死因子c(tumour necrosis factor-α,TNF-α)、诱导型一氧化氮合酶(inducible nitric oxide synthase、iNOS)、基质金属蛋白酶-9(matrix metalloproteinase-9,MMP-9).结果 实验组CGRP基因表达阳性,载体组β-半乳糖苷酶原位染色阳性.实验组内膜面积/中膜面积比值显著低于对照组和载体组.实验组MCP-1、TNF-α、iNOS、MMP-9显著低于对照组和载体组.结论 CGRP基因表达抑制了巨噬细胞浸润及炎症因子MCP-1、TNF-α、iNOS、MMP-9的表达,通过多种机制保护移植静脉,发挥防治移植静脉病的作用.“,”Objective To investigate the effect and mechanism of calcitonin gene-related peptide (CGRP) on the prevention and treatment of transplant vein graft disease.Methods The 25 New Zealand white rabbits were divided into three groups:an experimental group [n=8,the rabbit jugular veins transfected with adeno-associated virus vector tipe 2/1 containing CGRP gene (AAV2/1-CGRP)],a carrier group [n=9,transfected with mosaic adeno-associated virus vector tipe 2/1 containing LacZ gene (AAV2/1-LacZ)] and a control group (n=8,saline) and then the cervical veins were implantated into the ipsilateral carotid artery by reverse end-side anastomosis.At 4 weeks after surgery,the pathology of the specimens,CD68 immunohistochemistry,in situ β-galactosidase staining were obtained.The expression of CGRP gene was detected by reverse transcription-polymerase chain reaction (RT-PCR).Monocyte chemoattractant protein-1(MCP-1),tumour necrosis factor-α (TNF-α),inducible nitric oxide synthase (iNOS) and matrix metalloproteinase-9 (MMP-9) were detected by real-time polymerase chain reaction (real-time PCR).Results The CGRP and LacZ gene expression was positive at postoperative 4 weeks.The intima/media ratio was significantly inhibited in the experimental group.Macrophage infiltration and expression of inflammatory mediators including MCP-1,TNF-α,iNOS and MMP-9 were also significantly inhibited in the experimental group.Conclusion Transfection of AAV2/1-CGRP inhibits inflammatory mediator expression,macrophage infiltration and neointimal hyperplasia in experimental vein graft disease.