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目的通过胸骨上窝途径微创建立小鼠心衰模型。方法选用雄性昆明种系小鼠,经胸骨上窝利用钽夹部分夹闭主动脉弓,通过测量心脏体重比(HW/BW)、心脏超声以及组织学检查观察该方法创建小鼠心衰模型可行性。结果部分夹闭主动脉弓10周,HW/BW较假手术组明显增加(P<0.01);左室舒张期末直径(LVEDD)显著增大(P<0.01);左室后壁舒张期末厚度(LVPWD)明显变薄(P<0.01);射血分数(EF)以及短轴缩短率(FS)显著变小(P<0.01);夹闭部分主动脉弓10周后其血清氨基末端B型利钠肽前体(NT-pro BNP)较假手术组明显增加(P<0.01)。组织学观察左室部分心肌纤维排列紊乱、断裂,不同程度的结构破坏,心肌细胞呈不同程度变性及肥大,部分心肌细胞呈局灶性或片状坏死。结论经胸骨上窝途径部分夹闭主动脉弓建立小鼠心衰模型方法简单易行、微创、可靠。
Objective To establish a mouse model of heart failure by minimally invasive supraternal suture. Methods Male Kunming mice were selected. The aortic arch was partially occluded with tantalum clip through the suprasternal fossa. The feasibility of establishing the model of heart failure in mice was observed by measuring the ratio of heart to body weight (HW / BW), echocardiography and histology. RESULTS: After clamping the aortic arch for 10 weeks, HW / BW increased significantly (P <0.01), left ventricular end diastolic diameter (LVEDD) increased significantly (P <0.01), left ventricular posterior wall diastolic thickness (P <0.01). The ejection fraction (EF) and short axis shortening (FS) significantly decreased (P <0.01). After clipping the aortic arch for 10 weeks, the levels of serum amino-terminal B-type natriuretic peptide (NT-pro BNP) than the sham group (P <0.01). Histological observation Left ventricular myocardial fibers arranged in disorder, fracture, varying degrees of structural damage, myocardial cells were varying degrees of degeneration and hypertrophy, some myocardial cells were focal or necrosis. Conclusion The establishment of a mouse model of heart failure by clipping the aortic arch via the supraternal fossa approach is simple, minimally invasive, and reliable.