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目的 探讨 2型糖尿病 (T2DM)患者一氧化氮 (NO)、肿瘤坏死因子 浕(TNF 浕)及可溶性肿瘤坏死因子受体 (sTNF R)水平变化及意义。方法 NO采用硝酸还原酶法 ,TNF 浕、ｓＴＮＦ R采用双抗体夹心ELISA法。结果 T2DM患者血清NO水平显著性降低 (P <0 .0 1) ,NO水平与患者病程及病情呈负相关 ;血浆TNF 浕、ｓＴＮＦ R水平显著升高 (P <0 .0 1) ,随着病程的延长而升高 ,且有无并发症患者水平也相差显著 ,TNF 浕、ｓＴＮＦ R水平与患者病程及病情呈正相关。结论 检测T2DM患者NO、TNF 浕及ｓＴＮＦ R水平 ,对诊断病情、并发症的预测及治疗 ,可能有一定价值。
Objective To investigate the changes and significance of nitric oxide (NO), tumor necrosis factor (TNF) and soluble tumor necrosis factor receptor (sTNF R) in type 2 diabetes mellitus (T2DM) Methods NO nitrate reductase method, TNF 浕, sTNF R double antibody sandwich ELISA method. Results The level of serum NO in T2DM patients was significantly lower than that in T2DM patients (P <0.01). The level of NO was negatively correlated with the duration and severity of the disease. The levels of plasma TNF 浕 and sTNF R were significantly increased (P <0.01) Duration of disease increased and increased, and the level of patients with or without complications also significant difference, TNF 浕, sTNF R level and the patient’s duration and condition were positively correlated. Conclusion The detection of NO, TNF 浕 and sTNF R in T2DM patients may be of value in the diagnosis and treatment of the disease and complications.