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用美国普度大学M.Levy实验室提供的一个散布的重复序列探针(MGR—cPB586),对中国稻区田间分离的13个稻瘟病菌株DNA的EcoRI限制性片段长度多态性进行了研究。结果表明:稻瘟病菌不同生理小种间的RFLP普遍存在,MGR—DNA指纹能清楚地区分中国稻瘟病菌的主要生理小种,并能反映出同一生理小种内复杂的亲缘关系。
The EcoRI restriction fragment length polymorphism of the 13 M. grisea isolates isolated from rice fields in China was studied by using a scattered repeat probe (MGR-cPB586) provided by M. Levy Laboratory, Purdue University. The results showed that RFLP was common among different races of M. grisea. MGR-DNA fingerprinting could clearly distinguish the main races of M. grisea in China and could reflect the complex genetic relationship within the same race.