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采用原子力显微镜和衍射显微术,在纳米精确尺度探测副伤寒沙门氏菌B(Sp B)感染宿主红细胞(RBC)膜微观结构和力学特性,涉及细胞的形变、膜面内剪切模量和弯曲模量.结合这两种单分子测量技术,利用相关的数学模型表述RBC膜对菌体Sp B的入侵非常敏感.实验结果显示,不同感染期间的Sp B寄生菌体,能够引起宿主RBC膜结构改变,形变能力降低,膜剪切模量和弯曲模量显著增加.这些力学特性的变化影响RBC的输氧和循环功能.实验结果表明,Sp B具有独特的鞭毛调控系统,入侵的毒性菌体寄生蛋白与血影蛋白网络中的运输蛋白有特异结合位点,导致RBC膜骨架网络、波动力学和细胞内、外基质都产生应激反应,这有可能为理解Sp B感染RBC的发病机理和寄生途径提供一些新的实验思路和分析依据.
Atomic force microscopy and diffraction microscopy were used to detect the microstructure and mechanical properties of the RBC membrane of S. paratyphi B (Sp B) infected cells at the nanoscale scale. The changes of cell morphology, membrane in-plane shear modulus and bending mode .The combination of these two single-molecule measurement techniques, the use of mathematical models to describe the invasion of RBC membrane Sp B cells is very sensitive.The experimental results show that Sp B parasites during different infection can cause changes in the host RBC membrane structure , Deformation ability decreased, membrane shear modulus and flexural modulus increased significantly.The changes of these mechanical properties affected RBC oxygen transport and circulatory function.The experimental results show that Sp B has a unique flagellum regulatory system, the invasion of toxic bacterial parasitic protein Specific binding sites to transport proteins in the spectrin network result in stress responses in the RBC membrane scaffold network, wave kinetics, and intracellular and extracellular matrices, which may be useful in understanding the pathogenesis and parasitic pathways of SpBC-infected RBC Provide some new experimental ideas and analysis basis.