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目的探讨骨髓动员在糖尿病小鼠缺血肢体新生血管形成中的作用。方法采用链脲佐菌素建立糖尿病鼠模型,切除一侧肢体股动脉,注射粒细胞集落刺激因子(G-CSF)进行骨髓动员,以观察缺血肢体新生血管的形成。结果糖尿病小鼠模型建立8周后,血浆一氧化氮(NO)水平开始下降,骨髓动员后8 d,外周血CD~+_(34)细胞明显增加[从(0．15±0．05)%到(2．30±0．41)%],半定量RT-PCR检测缺血肢体肌肉组织的血管内皮细胞生长因子mRNA表达与正常对照组比较明显增强；动员后2周,激光多普勒血流检测骨髓动员组的缺血肢体血流明显高于未动员组[(76．37±6．10)%vs(18．07±3．40)%,P＜0．05],vWF免疫组化微血管密度检测动员组也明显高于未动员组[(26．6±4．8)个/每视野vs(11．5±2．6)个/每视野,P＜0．05]。结论糖尿病能降低小鼠血管内皮细胞的功能,但骨髓动员能促进糖尿病小鼠缺血肢体的新生血管形成。
Objective To investigate the role of bone marrow mobilization in neovascularization of ischemic limbs in diabetic mice. Methods Streptozotocin (STZ) was used to establish a diabetic mouse model. The femoral artery of one limb was excised and granulocyte colony stimulating factor (G-CSF) was injected to mobilize the bone marrow to observe the formation of neovascularization in ischemic limbs. Results After 8 weeks of establishment, the level of NO began to decline in diabetic mice. After 8 days of mobilization, the number of CD34 + cells in peripheral blood increased significantly (from (0.15 ± 0.05) % To (2.30 ± 0.41)%]. The expression of VEGF mRNA in ischemic limb muscle tissue was significantly increased by semi-quantitative RT-PCR compared with that of the normal control group. Two weeks after the mobilization, the laser Doppler The blood flow of ischemic limbs in the bone marrow mobilization group was significantly higher than that in the non-mobilized group [(76.37 ± 6.10)% vs (18.07 ± 3.40)%, P <0.05] The number of mobilized microvessel density test group was also significantly higher than that of the non-mobilized group [(26.6 ± 4.8) vs visual field vs (11.5 ± 2.6) per visual field, P <0.05). Conclusion Diabetes mellitus can reduce the function of vascular endothelial cells in mice, but bone marrow mobilization can promote neovascularization of ischemic limbs in diabetic mice.