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[Objectives] To analyze main components of polyphenols of Trillium tschonoskii Maxim. and study changes under different Se concentrations. [Methods] In this experiment,the Trillium tschonoskii Maxim. was used and spectrophotometry was applied to measure the total phenols in stems,leaves and rhizomes of Trillium tschonoskii Maxim. [Results]Total phenols in stems and leaves and rhizomes of Trillium tschonoskii Maxim. were 3. 026% and 0. 513% respectively,the content of total phenols in stems and leaves was 5. 9 times of that in rhizomes; total phenol extracting solution was analyzed through UV-Vis scanning,HPLC detection,and LC-MS; 3 main polyphenol compounds were obtained: chlorogenic acid,caffeic acid and rutin,in which chlorogenic acid and caffeic acid were firstly discovered in Trillium tschonoskii Maxim. and rutin was firstly discovered in stems and leaves of Trillium tschonoskii Maxim. HPLC method was used to measure the content of chlorogenic acid,caffeic acid,and rutin in stems and leaves of Trillium tschonoskii Maxim. under different Se concentrations. [Conclusions]The content of chlorogenic acid in stems and leaves of Trillium tschonoskii Maxim. increases with the increase of Se concentration,showing Se promotes Se accumulation and the maximum content of chlorogenic acid is 2. 58%; the content of caffeic acid in stems and leaves of Trillium tschonoskii Maxim. increases slightly with the Se concentration,showing the promotion of Se to accumulation of caffeic acid is not significant;the content of rutin in stems and leaves of Trillium tschonoskii Maxim. firstly increases then declines with the increase in Se concentration,at25 mg/kg Se concentration,rutin reached the maximum value,showing the promotion of Se to rutin accumulation is not significant.
[Objectives] To analyze main components of polyphenols of Trillium tschonoskii Maxim. And study changes under different Se concentrations. [Methods] In this experiment, the Trillium tschonoskii Maxim. Was used and spectrophotometry was applied to measure the total phenols in stems, leaves and rhizomes of Trillium tschonoskii Maxim. [Results] Total phenols in stems and leaves and rhizomes of Trillium tschonoskii Maxim. were 3. 026% and 0. 513% respectively, the content of total phenols in stems and leaves was 5. 9 times of that in rhizomes; total phenol solution solution was analyzed through UV-Vis scanning, HPLC detection, and LC-MS; 3 main polyphenol compounds were obtained: chlorogenic acid, caffeic acid and rutin, which chlorogenic acid and caffeic acid were initially discovered in Trillium tschonoskii Maxim. and rutin was found in stems and leaves of Trillium tschonoskii Maxim. HPLC method was used to measure the content of chlorogenic acid, caffeic acid, and rutin in stems a nd leaves of Trillium tschonoskii Maxim. under different Se concentrations. [Conclusions] The content of chlorogenic acid in stems and leaves of Trillium tschonoskii Maxim. increases with the increase of Se concentration, showing Se promotes Se accumulation and the maximum content of chlorogenic acid is 2. 58%; the content of caffeic acid in stems and leaves of Trillium tschonoskii Maxim. Increases slightly with the Se concentration, showing the promotion of Se to accumulation of caffeic acid is not significant; the content of rutin in stems and leaves of Trillium tschonoskii Maxim. initially increased then declines with the increase in Se concentration, at 25 mg / kg Se concentration, rutin reached the maximum value, showing the promotion of Sertin accumulation is not significant.