【摘 要】
：
Thaumatin-like protein (TLP) plays an important role in combating plant pathogen infection.Common root rot caused by Bipolaris sorokiniana and leaf rust caused by Puccinia triticina (Pt) are major fungal dis-eases in wheat.The disease responses of TaTLP1-
【机 构】
：
Technological Innovation Center for Biological Control of Crop Diseases and Insect Pests of Hebei Pr
论文部分内容阅读
Thaumatin-like protein (TLP) plays an important role in combating plant pathogen infection.Common root rot caused by Bipolaris sorokiniana and leaf rust caused by Puccinia triticina (Pt) are major fungal dis-eases in wheat.The disease responses of TaTLP1-overexpressing transgenic lines (TaTLP1-OE) were eval-uated after inoculation with each pathogen.The TaTLP1-OE lines had no apparent differences in tiller number and 1000-kernel weight from the wild type Jinan Wheat No.1 (JW1),whereas resistance to leaf rust and common root rot was improved,resulting from activated peroxidase and β-1,3-glucanase after B.sorokiniana infection,and reactive oxygen species-related genes were upregulated in TaTLP1-OE lines after Pt infection.These results indicated that stable expression of TaTLP1 increased resistance against both diseases.
其他文献
应激能够造成动物脑部、心血管系统、呼吸系统、消化系统等方面的功能紊乱与结构受损,严重影响动物健康.核因子-κB(nuclear factor-kappa B,NF-κB)作为重要的蛋白转录调节因子,在动物机体应激反应及相关疾病发生过程中起着重要的作用.现将从应激对动物机体的影响,NF-κB在其中的作用等方面进行综述,为今后对减少应激损伤的研究提供理论依据.
旨在探讨酶解修饰对猴头菇多糖(Hericium erinaceus polysaccharide,HEP)结构特征及其免疫活性的影响.采用内切-1,5-α-阿拉伯聚糖酶对HEP进行酶解,通过高效凝胶色谱法、红外光谱法等对HEP与酶解猴头菇多糖(enzymatic hydrolysis of Hericium erinaceus polysaccharide,EHEP)的结构进行对比分析,并利用MTT法、流式细胞术和Griess法比较了HEP与EHEP对RAW 264.7细胞的免疫调节作用.结果 显示,EH
旨在研究不同非纤维性碳水化合物(NFC)/中性洗涤纤维(NDF)饲粮对藏羊肉中营养成分、氨基酸和脂肪酸含量的影响.采用单因素随机设计,以高原型藏羊为研究对象,将30只体质量为(26.38±0.43) kg健康的藏羔羊,按性别分为3个公羊组和3个母羊组,每组5只,分别饲喂NFC/NDF为2.20(精粗比为70∶30)、1.39(精粗比为50∶50)和0.92(精粗比为30∶70)的试验饲粮.预试期为15d,正试期为90 d.试验结束后,每组选取3只试验羊进行屠宰,采集第12～13肋骨间背最长肌,测定其营养成
比较白针、水针、穴位埋线3种针术刺激后海穴(GV-1)对便秘大鼠的作用及其对肠神经递质及5-羟色胺(5-HT)信号途径的影响.选用SD大鼠60只,随机分为A～F组(对照组、模型组、西药组、白针组、水针组、埋线组),共6组,每组10只.除空白组外,其余各组大鼠每天灌胃盐酸洛哌丁胺,建立便秘模型,盐酸洛哌丁胺一直用至试验结束以维持便秘.便秘6d,C组应用1.3 mg/(kg·d)莫沙必利灌胃,D组针刺GV-120 min,E组GV-1注射0.1 mL VB12,F组GV-1埋入羊肠线.除F组仅处理1次外,其余
旨在研究母鼠长期铜暴露对子代大鼠睾丸功能的影响.将24只健康雌性SD大鼠随机分为A、B、C3组,每组8只,A、B、C组分别饲喂AIN-93G基础日粮、AIN-93G基础日粮+CuSO4120 mg/kg、AIN-93G基础日粮+CuSO4240 mg/kg 3种不同铜水平日粮,试验期8周.雌鼠与性成熟雄性SD大鼠交配受孕后,在妊娠期和哺乳期继续铜覆盖.断乳后饲喂AIN-93G基础日粮,待子代雄性大鼠10周龄后,每组随机取8只,分别标记为A0、B0、C0组,记录体质量及睾丸、附睾脏器指数、检测睾丸组织铜水平
Brassica napas L.(rapeseed) is one of the most important oil crops with large cultivated area in China.Seed size and seed weight play crucial roles for yield and harvest.In this study,a type of 15 bp-deletion in BnaGRF7.C02 coding region was identified th
Two potential BRASSINAZOLE RESISTANT 1 (BZR1) homologs were downregulated by brassinosteroids(BRs) in Setaria italica roots.Functional analysis showed that BR regulates the dephosphorylation and nuclear localization of SiBZR1 and that SiBZR1 binds conserv
Chloroplasts are the center of plant life activities including photosynthesis,growth and development,and abiotic stress response.Chloroplast development and biogenesis in rice have been studied in detail,but how does abiotic stress affect chloroplasts is
为研究microRNA在致细胞病变型牛病毒性腹泻病毒(cpBVDV)感染过程中对病毒复制和调控炎症反应的作用机制,本研究以MDBK细胞为模型,通过对cpBVDV感染MDBK前后的总RNA进行microRNA芯片反应,筛选与炎症反应相关的显著差异性microRNA,并预测出其靶基因.通过将miR-2904转染至MDBK细胞,探索其在BVDV感染MDBK细胞过程中的调控作用,并验证其靶基因;利用qRT-PCR检测miR-2904、BVDV 5\'UTR及相关炎症因子mRNA的表达水平,利用Western
Rice,a major staple,is the most salt-sensitive cereal.High salinity triggers several adaptive responses in rice to cope with osmotic and ionic stress at the physiological,cellular,and molecular levels.A major QTL for salinity tolerance,named Saltol,is pre