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热应激蛋白70ku（heatstressprotein，HSP70）是主要的热应激蛋白。用已纯化的HSP70抗原和已制备好的HSP70抗体，采用十二烷基磺酸钠聚丙烯酰胺凝胶电泳的高分辨率和抗原抗体反应的特异性相结合的免疫印迹法（westernblot）检测17名高温作业工人和17名对照组人群血清中HSP70抗原和抗体。结果，与对照组相比，高温作业工人血清中HSP70含量增加，HSP70抗体阳性率为23.5％。结果表明westernblot法用于检测工人血清中HSP70抗原和抗体是可行的。
Heat stress protein 70ku (heatstressprotein, HSP70) is the main heat-stress protein. Western blot was performed using the purified HSP70 antigen and the prepared HSP70 antibody in combination with the high resolution of the sodium dodecyl sulfate polyacrylamide gel electrophoresis and the specificity of the antigen-antibody reaction High-temperature workers and 17 control group serum HSP70 antigen and antibody. As a result, compared with the control group, the HSP70 content in the serum of workers exposed to high temperature increased, and the positive rate of HSP70 antibody was 23.5%. The results show that western blot method for the determination of worker serum HSP70 antigen and antibody is feasible.