Expression of cyclooxyenase-2 protain and its relationship with HIF-1α in HCC

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Objective: To investigate the clinical significance of COX-2 (Cyclooxygenase-2) expression in HCC (Primary hepatocellular carcinoma) and clarify whether COX-2 is correlated with hypoxia-inducible factor-1α (HIF-1α) in the development of HCC. Methods: Tumor tissues were obtained from 53 patients with HCC. COX-2 and HIF-1α were determined by immunohistochemistry. All 53 patients were regularly followed up and the data were collected prospectively. Results: Immunostaining showed the expression of COX-2 (n=33, 62.3%) and HIF-1α (n=36, 67.9%) in most tumor cells. The level of COX-2 was correlated with HIF-1α levels(r=0.440,P<0.01). There were significant correlation between clinicopathological features and higher tumor cytosolic COX-2 level was in the presence of multiple tumors (P=0.01), venous invasion (P=0.03), advanced tumor stage (P=0.01), and well-different tumor grade (P=0.03). High tumor cytosolic COX-2 level was correlated with patient’s worse prognosis (P=0.0085). Conclusion: Elevated tumor COX-2 level is correlated with elevated HIF-1α levels and invasiveness in HCC, suggesting COX-2 plays an important role in the progression of HCC,and may be an important therapeutic target in HCC. Objective: To investigate the clinical significance of COX-2 (Cyclooxygenase-2) expression in HCC (Primary hepatocellular carcinoma) and clarify whether COX-2 is correlated with hypoxia-inducible factor-1α (HIF-1α) in the development of HCC. Methods: Tumor tissues were obtained from 53 patients with HCC. COX-2 and HIF-1α were determined by immunohistochemistry. All 53 patients were regularly followed up and the data were collected prospectively. Results: Immunostaining showed the expression of COX-2 (n (N = 36, 67.9%) in most tumor cells. The level of COX-2 was correlated with HIF-1α levels (r = 0.440, P <0.01) between clinicopathological features and higher tumor cytosolic COX-2 level was in the presence of multiple tumors (P = 0.01), venous invasion (P = 0.03), advanced tumor stage (P = 0.01), and well- 0.03). High tumor cytosolic COX-2 level was correlated with patient’s worse prognosis (P = 0.0085) Conclusio n: Elevated tumor COX-2 level is correlated with elevated HIF-1α levels and invasiveness in HCC, suggesting COX-2 plays an important role in the progression of HCC, and may be an important therapeutic target in HCC.
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