论文部分内容阅读
目的探讨生命早期经饲料补充维生素D对链脲菌素作用小鼠空腹血糖和胰岛素水平,糖尿病发病率,以及链脲菌素致胰岛损伤的影响。方法 C57BL/6鼠离乳后分别饲喂标准饲料和补充维生素D(VD)饲料9周后注射链脲菌素(STZ),继续喂养4周,注射STZ后每周末测定空腹血糖,计算糖尿病发病率,4周后测血清胰岛素水平,并采用HE染色法观察胰腺形态学变化。结果①生命早期补充VD能降低STZ作用小鼠的空腹血糖水平,并以补充高剂量VD的降糖效果最好,其在注射STZ后4周血糖值明显低于糖尿病模型组(P<0.01);②生命早期补充VD降低STZ致小鼠糖尿病发病率,并以补充高剂量VD的干预效果最好,其在注射STZ后4周时糖尿病发病率为0%,完全抑制糖尿病的发病(P<0.01);③生命早期补充高剂量和中剂量VD明显上调STZ作用小鼠的血清胰岛素水平(P<0.05),并以补充高剂量VD的上调作用最显著(P<0.01)。④生命早期补充高剂量和中剂量VD对STZ致胰岛损伤有明显保护作用,且以补充高剂量VD的保护作用最好。结论生命早期补充一定量VD能降低STZ作用小鼠的血糖水平和糖尿病发病率,上调血清胰岛素水平,改善STZ对胰岛细胞的损伤,且均以补充高剂量VD的作用最显著。
Objective To investigate the effects of dietary vitamin D supplementation on the fasting blood glucose and insulin levels, the incidence of diabetes and streptozotocin-induced pancreatic islet injury induced by streptozotocin in early life. Methods C57BL / 6 mice were fed with standard diet and vitamin D (VD) feed for 9 weeks, respectively. STZ was injected for another 4 weeks. After the injection of STZ, fasting blood glucose (FBG) was measured every weekend to calculate the incidence of diabetes Rate, serum insulin levels after 4 weeks, and the morphological changes of the pancreas were observed by HE staining. Results ① VD supplementation in early life reduced the level of fasting blood glucose in STZ-treated mice, and the hypoglycemic effect of high-dose VD was best. The blood glucose level at 4 weeks after STZ injection was significantly lower than that of diabetic model group (P <0.01) ; ② Early VD supplementation reduced the incidence of STZ-induced diabetes in mice and was best supplemented with high-dose VD. The incidence of diabetes was 0% at 4 weeks after STZ injection, with a complete suppression of the onset of diabetes (P < 0.01). ③In early life, supplementation of high dose and middle dose of VD significantly up-regulated serum insulin level in STZ-treated mice (P <0.05), and up-regulated the effect of high dose of VD. ④ early life supplementation of high dose and medium dose of VD on STZ-induced islet injury had significant protective effect, and to supplement the best protection of high-dose VD. CONCLUSION: Adding a certain amount of VD early in life can reduce the blood sugar level and the incidence of diabetes in STZ-treated mice, increase the level of serum insulin, and improve the damage of STZ to islet cells. Both of them have the most significant effect of supplementing high-dose VD.