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用琼脂糖凝胶电泳、电镜等方法研究大肠杆菌脂多糖（ＬＰＳ）和氢化考的松所致的小鼠胸腺细胞凋亡，以建立适于实验的细胞凋亡模型，并确定了凋亡模型的最佳用药剂量和时相。实验结果表明，ＬＰＳ和氢化考的松均可诱导小鼠胸腺细胞发生凋亡，ＬＰＳ腹腔注射量为２５μｇ／只时造模较为适宜，造模最佳时相为腹腔注射１８ｈ，氢化考的松模型经测定其最佳时相为肌肉注射２４ｈ。两种凋亡模型比较，ＬＰＳ模型造模时间短（仅需１８ｈ），简便易行，是一种较好的研究细胞凋亡的实验工具。
The apoptosis of thymocytes induced by lipopolysaccharide (LPS) and cortisone of hydrocortisone was studied by agarose gel electrophoresis and electron microscopy to establish an apoptosis model suitable for the experiment and to determine the apoptosis model The best dosage and time phase. The experimental results showed that LPS and hydrocortisone could induce the apoptosis of thymocytes in mice. When LPS was injected intraperitoneally at a dosage of 25μg / mouse, the best model was intraperitoneal injection for 18h, and hydrocortisone The model was determined the best time for intramuscular injection 24h. Compared with the two apoptosis models, LPS model has a short modeling time (only 18h), which is simple and easy to use. It is a good experimental tool to study apoptosis.