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为探讨高脂膳食对家兔血清过氧化脂质（ＬＰＯ）升高和ＬＤＬ、ＶＬＤＬ，特别是ＨＤＬ氧化修饰的影响。正常对照组（ｎ＝８）喂以普通饲料，高脂实验组（ｎ＝８）用高胆固醇饲料（普通饲料加５％猪油，另每只兔加喂０．５ｇ胆固醇）喂养１２周。兔血清ＬＤＬ、ＶＬＤＬ及ＨＤＬ用密度梯度超速离心法分离得到。ＬＤＬ、ＶＬＤＬ及ＨＤＬ的氧化修饰用琼脂糖凝胶电泳、２３４ｎｍ光吸收及ＴＢＡＲＳ荧光检测进行鉴定。结果显示：高脂实验组兔血清的ＴＣ、ＴＧ和ＬＰＯ均显著高于正常对照组（Ｐ＜０．０１），而ＨＤＬ－Ｃ则显著降低（Ｐ＜０．０５）；同时，高脂实验组的ＬＤＬ、ＶＬＤＬ和ＨＤＬ的电泳迁移率增加，其２３４ｎｍ光吸收及ＬＰＯ含量均显著高于正常对照组（Ｐ＜０．０１）。表明高脂膳食在诱发家兔血清ＴＣ、ＴＧ显著增高的同时，不仅可引起血浆ＬＤＬ及ＶＬＤＬ的氧化修饰，而且还可引起ＨＤＬ在活体内的氧化修饰
To investigate the effect of high-fat diet on the increase of serum lipid peroxides (LPO) and the oxidative modification of LDL, VLDL, especially HDL in rabbits. The normal control group (n = 8) was fed with normal diet and the high fat diet group (n = 8) fed with high cholesterol diet (normal diet plus 5% lard and 0.5g cholesterol per rabbit) for 12 weeks. Rabbit serum LDL, VLDL and HDL density gradient ultracentrifugation obtained. The oxidative modification of LDL, VLDL and HDL was identified by agarose gel electrophoresis, 234 nm light absorption and TBARS fluorescence detection. The results showed that the levels of TC, TG and LPO in serum of high-fat diet group were significantly higher than those of normal control group (P <0.01), but HDL-C was significantly lower (P <0.05) The electrophoretic mobility of LDL, VLDL and HDL increased, and the absorption and LPO content at 234 nm were significantly higher than that of the normal control group (P <0.01). The results showed that high fat diet induced rabbit serum TC, TG was significantly increased at the same time, not only can cause plasma LDL and VLDL oxidative modification, but also can cause HDL in vivo oxidative modification