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近十多年来,免疫学发展很快,对体液免疫球蛋白之结构、功能、定量方法,都有很大进展,对免疫性疾病也有了进一步的认识。我们为了探讨血栓闭塞性脉管炎与血液免疫球蛋白的关系,对58例患者血液IgG、IgA、IgM进行了定量测定,以求对本病有进一步的了解,提高防治水平。材料和方法 1.提纯人IgG、IgA、IgM。 2.免疫动物,制备三种单价抗血清。 3.取患者空腹血,用单向免疫扩散法,分别进行Ig半定量。结果 1.以100例献血员混合血清作为抗原标准作正常值对照。 2.58例脉管炎患者的血液Ig测定情况见表。
In the recent ten years, the development of immunology has been rapid. The structure, function and quantitation of humoral immunoglobulin have made great progress, and further understanding of immune diseases has also been made. We in order to explore the relationship between thromboangiitis obliterans and blood immunoglobulin, 58 patients with blood IgG, IgA, IgM quantitative determination, in order to further understand the disease, improve the level of prevention and treatment. Materials and Methods 1. Purification of human IgG, IgA, IgM. 2. Immunize animals and prepare three monovalent antiserums. 3. Take the patient fasting blood, with one-way immunodiffusion Ig semi-quantitative. 100 cases of blood donors mixed serum as antigen standard for normal control. 2.58 cases of vasculitis in patients with blood Ig measurement table.