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检测急、慢性乙型肝炎患者血清IL-10和IFN-γ水平,探讨乙型肝炎慢性化的机理。方法:用双抗夹心ELISA法定量检测25例急性乙肝,35例慢性乙肝和30例正常人血清IL-10和IFN-γ水平。结果:慢性乙肝患者血清IL-10浓度明显高于急性乙肝患者(P<0.01),且高于正常对照组(P<0.01)。而慢性乙肝组患者血清IFN-γ浓度明显低于急性乙肝组(P<0.01),两组的血清IFN-γ浓度均明显高于正常对照组(P<0.01)。慢性乙肝患者血清IFN-γ浓度与ALT有相关性。结论:慢性乙肝患者IL-10水平增高,限制了IFN-γ合成,削弱了其对HBV清除作用,可能是乙型肝炎慢性化机理之一;慢性乙肝患者血清IFN-γ浓度与ALT有相关性。
Serum levels of IL-10 and IFN-γ in patients with acute and chronic hepatitis B were measured to explore the mechanism of chronic hepatitis B. Methods: Serum levels of IL-10 and IFN-γ in 25 cases of acute hepatitis B, 35 cases of chronic hepatitis B and 30 normal persons were detected by double-antibody sandwich ELISA. Results: Serum IL-10 level in patients with chronic hepatitis B was significantly higher than that in patients with acute hepatitis B (P <0.01), and higher than that in the control group (P <0.01). Serum IFN-γ levels in patients with chronic hepatitis B were significantly lower than those in patients with acute hepatitis B (P <0.01). Serum IFN-γ levels in both groups were significantly higher than those in controls (P <0.01). Serum levels of IFN-γ in patients with chronic hepatitis B are associated with ALT. Conclusion: Increased levels of IL-10 in chronic hepatitis B patients limit the synthesis of IFN-γ, impaired HBV clearance and may be one of the mechanisms of chronic hepatitis B. Serum IFN-γ levels are associated with ALT in patients with chronic hepatitis B .