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以青龄大鼠（３月龄）为对照组，采用开场行为和一次被动回避反应模型，观察了老龄大鼠（２６月龄）自发活动与记忆保持的行为表现，同时使用荧光指示剂Ｆｕｒａ－２／ＡＭ与Ｔｂ３＋检测了各主要脑区（前皮层、海马、间脑、小脑）突触体内游离钙与膜结合钙水平，与对照组进行对比分析。实验结果表明：老龄鼠在新异环境中探究反应减弱，自发活动减少；老龄鼠对伤害性刺激（电击）的记忆保持能力衰退；在行为表现发生变化的同时，伴随有脑突触体钙水平的改变：海马、皮层和间脑３个脑区的突触体游离钙水平均显著升高，尤以海马升高为著（Ｐ＜０．０１），同时海马与小脑的突触体膜结合钙也极显著增加（Ｐ＜０．０１）。鉴于海马是与记忆密切相关的脑区，老龄鼠海马突触体内游离钙与膜结合钙都极显著增加，呈现严重的钙超载状态，很可能正是记忆衰退的物质基础。
The behavior of spontaneous activity and memory retention in aged rats (26 months old) was observed by using open-ended behavior and passive avoidance response in aged rats (3 months old) as the control group. Fluorescent indicator Fura- 2 / AM and Tb3 + detected the intracellular free calcium and membrane bound calcium levels in the synapses of all major brain regions (prefrontal cortex, hippocampus, diencephalon and cerebellum), compared with the control group. The experimental results showed that the aging mice weakened in exploring reaction and spontaneous activity in neonatal environment, the memory retention ability of aging rats deteriorated to noxious stimuli (electric shock), accompanied by changes of behavioral manifestations accompanied with calcium levels of brain synaptosomes : Hippocampus, cortex and interneurons in three brain regions of synaptosome free calcium levels were significantly increased, especially in hippocampus (P <0.01), while the hippocampus and cerebellar synaptic membrane binding Calcium also increased significantly (P <0.01). In view of the hippocampus is closely related to memory brain area, hippocampal hippocampal synapses in old calcium and membrane bound calcium were significantly increased, showing a serious calcium overload status, it is likely the material basis for memory loss.