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目的用脉冲场凝胶电泳(PFGE)分型技术,研究本区2013年肠道多病原监测标本中的沙门菌分子流行病学,查找沙门菌污染源,为预测疫情和制定防治措施提供依据。方法选择XbaⅠ酶对14株沙门菌全基因组DNA进行酶切,用PFGE技术对菌株进行分子分型,Bionumerisc统计软件聚类分析。用Kirby-Bauer法(KB)进行药敏试验,分析沙门菌耐药情况及同源性分析。结果沙门菌用XbaⅠ限制性酶切后,分成14个PFGE图谱类型。虽然表现出较大的遗传多样性,但部分菌株有相关性。14株受试菌株对绝大部分常用的头孢类抗生素敏感,但个别菌株对青霉素类、四环素、复方新诺明、庆大霉素产生了耐药。结论 PFGE分子分型与传统实验室检测技术紧密结合可增强沙门菌的溯源和预警。
Objective To investigate the molecular epidemiology of Salmonella in the multi-pathogenic intestinal tract surveillance samples in 2013 in this district by using pulsed-field gel electrophoresis (PFGE) typing and to search for the sources of Salmonella, so as to provide the basis for forecasting the epidemic situation and making prevention and control measures. Methods Whole genome DNA of 14 strains of Salmonella was digested by Xba Ⅰ enzyme, and molecular typing was performed by PFGE. Cluster analysis was performed by Bionumerisc statistical software. Kirby-Bauer method (KB) for drug susceptibility testing, analysis of Salmonella resistance and homology analysis. Results Salmonella was digested with Xba I and divided into 14 PFGE patterns. Although showing greater genetic diversity, some strains are related. Fourteen tested strains were sensitive to the most commonly used cephalosporins, but individual strains were resistant to penicillins, tetracycline, cotrimoxazole and gentamicin. Conclusion The combination of PFGE molecular typing with traditional laboratory testing techniques can enhance the traceability and early warning of Salmonella.