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以叶用芥菜的子叶为外植体,对愈伤组织诱导和细胞悬浮培养的技术体系进行了研究.结果表明,愈伤组织诱导的最适培养基为MS+0.3mg/LNAA+1.0mg/L6-BA+0.1mg/L2,4-D,愈伤组织最佳增殖培养基为MS+3.0mg/L6-BA+1.0mg/L2,4-D.悬浮培养过程中细胞生长曲线呈S型,培养2～10d达对数生长期.将小细胞团接种于MS+1.0mg/LNAA+3.0mg/L6-BA分化培养基上,继代两次后,分化出芽,分化芽在培养基MS+0.1mg/LNAA上诱导生根,形成小植株.
The cotyledons of mustard leaf were used as explants to study the technology system of callus induction and cell suspension culture.The results showed that the optimal medium for callus induction was MS + 0.3mg / L NAA + 1.0mg / L6-BA + 0.1mg / L 2,4-D, the best proliferation medium for callus was MS + 3.0mg / L 6-BA + 1.0mg / L 2,4-D.The cell growth curve in suspension culture showed S type , And cultured for 2 ~ 10 days up to logarithmic growth phase.The small cell mass was inoculated on MS + 1.0mg / L NAA + 3.0mg / L 6-BA differentiation medium and subculture twice, + 0.1mg / LNAA induced rooting, the formation of plantlets.