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Objective:To establish the polytransgenic mice expressing the human HT and complement regulatory proteins (CRPs) and discuss their ability to resist the hyperacute rejection (HAR) and delayed xenograft rejection (DXR) of heterogenic transplantation. Methods:Transgenic mice were produced by microinjection to construct gene for human HT, delay acceleration factor (DAF) and/or CD59 into the male pronucleus of zygote. PCR and South blot were used to screen the positive transgenic mice.Flow cytometry (FCM) was used to detect the expression of HT, α-Gal and DAF or CD59 on the PBMCs of transgenic mice. The survival time and function of the heart oftransgenic mice were determined by a modified Langendorff cardiac perfusion appratus. The change of proteinosis on IgM、IgG、C3c and C9 from different cardiac vascular endothelial cells of transgenic mice were detected by immunohistochemistry. Results:HT,DAF or CD59 were highly expressed on the positive transgenic mice by FCM. The deposition of IgM、IgG、C3c or C9 in the cardiac vascular endothelial cells of the positive transgenic mice were decreased. The survival time and function of the heart of the co-transgenic mice with AB serum perfusion were significantly longer and higher than that of the single HT positive transgenic mice(P ＜0.05 ).Conclusion :The mice co-expressing HT/DAF or HT/CD59 could resist the HAR,which was better than those expressing HT alone.It is feasible to use HT and CRPs co-transgenic methods to resist the HAR and DXR.