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Non-radioactive ECL direct nucleic acid labelling and detection system was used for RFLP analysis inrice. The system involves directly labelling DNA or RNA probe with the enzyme horseradish peroxidase (HRP),and the detection of non-radioactive signal is based on chemiluminescence , that is the generation of light via enzyme(HRP)-catalyzed reactions. This is a simple, safe and quick method. The band patterns were clear and comparablewith those by ~32P labelling.
Non-radioactive ECL direct nucleic acid labeling and detection system was used for RFLP analysis inrice. The system Guides directly directly labeled DNA or RNA probe with the enzyme horseradish peroxidase (HRP), and the detection of non-radioactive signals is based on chemiluminescence, that is the generation of light via enzyme (HRP) -catalyzed reactions. This is a simple, safe and quick method. The band patterns were clear and comparablewith those by ~ 32P labelling.