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目的研究冈田酸(蛋白磷酸酶2A抑制剂,PP2A)抑制胰腺癌细胞株PANC-1迁移的过程并研究其增值机制。方法把PANC-1细胞分成等量的4组:对照组,实验-Ⅰ组、实验-Ⅱ组、实验-Ⅲ组,对照组不作处理,实验-Ⅰ组:用冈田酸处理,实验-Ⅱ组用Wnt/β-catenin通路抑制剂(FH535)处理,实验-Ⅲ组用冈田+FH535联合处理。用显微拍照计量面积方法来评估细胞迁移率,用面板吸光比率来评估细胞活性。结果实验-Ⅰ组和对照组在2,4,6 h的迁移率分别为16.28%,18.13%;21.94%,25.34%;27.67%,70.64%,实验-Ⅰ组在2,4,6 h这3个时间点的迁移率均明显低于对照组,差异有统计学意义(均P<0.05),表明冈田酸对胰腺癌细胞株PANC-1的迁移能力有抑制作用。实验-Ⅱ组与实验-Ⅲ组细胞活性在0,6,12,18 h分别为52.39%,51.81%;49.57%,30.67%;30.64%,12.57%;15.91%,3.59%,2组这4个时间点的细胞活性均明显低于对照组,差异有统计学意义(均P<0.05),表明冈田酸对胰腺癌细胞中Wnt/β-catenin通路的活性有下调作用;冈田酸对胰腺癌细胞株迁移及增殖的抑制作用被Wnt/β-catenin通路抑制剂削弱,可得出冈田酸对胰腺癌细胞株PANC-1迁移及增殖的抑制作用有可能基于Wnt/β-catenin信号通路。结论冈田酸之所以能够抑制胰腺癌细胞株PANC-1的迁移及增殖,其机制可能是对Wnt/β-catenin信号通路的活性抑制下调。
Objective To study the inhibitory effect of Okadaic acid (a phosphatase 2A inhibitor, PP2A) on the migration of pancreatic cancer cell line PANC-1 and to investigate its mechanism of added value. Methods PANC-1 cells were divided into 4 equal groups: control group, experimental -Ⅰgroup, experimental -Ⅱgroup, experimental -Ⅲgroup, and control group without treatment. ExperimentⅠgroup: Okadaic acid treatment, Groups were treated with Wnt / β-catenin pathway inhibitor (FH535), and experimental-group Ⅲ was treated with Okada + FH535. Cell migration was assessed using a microscopic photometric area method and cell viability was assessed using the panel absorbance ratio. Results In the experimental group I, the migration rates of group I and control were 16.28%, 18.13%, 21.94%, 25.34%, 27.67% and 70.64% respectively at 2, 4 and 6 h The migration rates at 3 time points were significantly lower than those in the control group (all P <0.05), indicating that okadaic acid can inhibit the migration of pancreatic cancer cell line PANC-1. The cell viability in experimental -Ⅱgroup and experimental -Ⅲgroup were 52.39%, 51.81%, 49.57%, 30.67%, 30.64%, 12.57%, 15.91%, 3.59% (P <0.05), indicating that Okadaic acid had a down-regulation effect on the activity of Wnt / β-catenin pathway in pancreatic cancer cells; Okadaic acid The inhibitory effect of okadaic acid on the migration and proliferation of pancreatic cancer cell line PANC-1 was attenuated by Wnt / β-catenin pathway inhibitor. It is concluded that the inhibitory effect of okadaic acid on the migration and proliferation of pancreatic cancer cell line PANC-1 may be based on the signal of Wnt / β-catenin path. Conclusion Okadaic acid can inhibit the migration and proliferation of pancreatic cancer cell line PANC-1, and its mechanism may be down-regulation of Wnt / β-catenin signaling pathway.