论文部分内容阅读
从柿疯病株的1—2年生枝条或根的输导组织中,抽取组织液并离心富集制备样品,经磷钨酸钠负染,和Epon812树脂常规包埋超薄切片,在电镜下检查很多多型性原核细胞,大小不一,长580—1600nm,宽510—1060nm,胞壁呈山脊状波纹,厚约25nm,无鞭毛而周身分布微小疣突,按其形状大小和亚显微结构特征确定为难养细菌(Fostidious bacteria)而不同于一般植物病原细菌,健株制备的样品中未检查到任何微生物。这个结果与前文报道青霉素可抑制和缓解柿疯病症状表现的结果是一致的[1],检查到的这种难养细菌认为是柿疯病的病原物。前已证明,柿疯病可以嫁接传病,并对该病的症状作了系统的观察和描述[1],本文报道柿疯病病原的研究结果。
Tissue fluid was drawn from the branches or roots of 1 - 2 year-old branches of persimmon-mad strains and centrifuged for enrichment to prepare samples. The samples were negative-stained with sodium phosphotungstate, and ultrasonically sliced with Epon812 resin, examined under electron microscope Many polymorphic prokaryotic cells of different sizes, long 580-1600nm, 510-1060nm wide, ridged ridged cell wall thickness of about 25nm, no flagella and peripheral distribution of small warts, according to their shape and size and sub-microstructure The characteristic was identified as Fostidious bacteria, which was different from the common plant pathogenic bacteria, and no microorganisms were detected in the samples prepared from the healthy plants. This result is consistent with the previous report that penicillin inhibits and relieves the symptoms of persimmon madness [1] and that this difficult to support bacterium was found to be the causative agent of persimmon madness. It has been previously demonstrated that persimmon can be grafted disease, and the symptoms of the disease were systematically observed and described [1], this paper reports the results of persimmon pathogens.