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利用Charleston(♀)×东农594(♂)的F14和F15代永久自交系群体154个单株后代,在2年3点条件下用气相色谱法测得其籽粒5种脂肪酸的含量,利用Win QTL Cartographer2.5复合区间作图法(CIM)进行QTL分析。结果共检测到47个相关的QTL,分布在13个连锁群上。多年多点同时检测到的QTL共有15个,其中控制软脂酸性状的2个,包括qPal-C2-2和qPal-A1-1;控制硬脂酸性状的4个,包括qSt-B1-1、qSt-B1-2、qSt-D1a-1和qSt-C2-1;控制油酸性状的3个,包括qOle-B2-1、qOle-G-1和qOle-H-1;控制亚油酸性状的有2个,包括qLin-C2-1和qLin-H-1;控制亚麻酸性状的4个,包括qLino-B1-1、qLino-C2-1、qLino-D1b-1和qLino-J-1。这些QTL的一致性较高,为特异脂肪酸含量标记辅助育种奠定了基础。大豆脂肪酸含量的主效QTL数量不多,效应大的不多,可能还受许多未能检测出来的微效基因控制。
154 offspring of F14 and F15 permanent inbred lines from Charleston × Dongnong 594 (♂) were used to determine the content of five fatty acids in kernels at 3:00 of 2 years under the conditions of Win QTL Cartographer 2.5 complex interval mapping (CIM) for QTL analysis. A total of 47 related QTLs were detected and distributed on 13 linkage groups. A total of 15 QTLs detected at the same time for many years, including 2 palmitate traits, including qPal-C2-2 and qPal-A1-1, and 4 stearic acid traits, including qSt-B1-1 , QSt-B1-2, qSt-D1a-1 and qSt-C2-1; controlling 3 oleic acid traits including qOle-B2-1, qOle-G-1 and qOle-H- 1; Two of them, including qLin-C2-1 and qLin-H-1, controlled four linolenic traits including qLino-B1-1, qLino-C2-1, qLino-D1b- 1. These QTLs are highly consistent and lay the foundation for marker-assisted breeding of specific fatty acid content. Soybean fatty acid content of the major effects of QTL number is small, the effect of large, may also be many failed to detect the micro-gene control.