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目的 研究人非小细胞肺癌细胞膜钾离子通道特性。方法 采用原代培养的非小细胞肺癌细胞、肺部良性病变和癌旁支气管上皮细胞 ,应用膜片钳全细胞记录方式检测细胞膜钾离子通道特性。结果 ( 1)非小细胞肺癌细胞膜K+ 通道表达的K+ 电流具有电压依赖性 ,能被K+ 通道阻断剂TEA阻断。鳞癌细胞膜电流具有失活趋势和内向整流特点 ;腺癌细胞膜电流具有不失活特性 ,无内向整流特点。TP =5 0～ 90mV时 ,时间常数 (τ值 )为 3.6～ 9.8ms。非小细胞肺癌细胞膜电容为 ( 15 .35± 0 .65 )pF ,K+ 电流密度为 ( 12 1.0 8±8.35 )A/F。 ( 2 )非小细胞肺癌细胞膜K+ 电流幅度、电流密度和膜电容较支气管上皮细胞明显增高 (P <0 .0 0 1) ,癌细胞τ值显著小于支气管上皮细胞的τ值 (P <0 .0 0 1)。结论 ( 1)非小细胞肺癌细胞膜存在与延迟整流性类似的钾离子通道 ,且表达明显增加 ,活动明显增强。 ( 2 )检测肺癌细胞膜K+ 通道可为研究肺癌发生、发展、侵袭和转移的信号传导提供理论基础和实验依据
Objective To study the potassium ion channel characteristics of human non-small cell lung cancer (NSCLC). Methods Primary cultured non-small cell lung cancer cells, benign pulmonary lesions, and para-cancerous bronchial epithelial cells were used. Patch-clamp whole-cell recording was used to detect the characteristics of cell membrane potassium channels. Results (1) K+ currents expressed in the K+ channels of non-small cell lung cancer cells were voltage-dependent and could be blocked by the K+ channel blocker TEA. The squamous cell carcinoma cell membrane current has the trend of inactivation and inward rectification; the membrane current of adenocarcinoma cells has no deactivation characteristics and no inward rectification. When TP = 50 ~ 90mV, the time constant (τ value) is 3.6 to 9.8ms. The cell membrane capacitance of non-small cell lung cancer was (15.35±0.55)pF, and the K+ current density was (12 1.0 8±8.35 )A/F. (2) The K+ current amplitude, current density and membrane capacitance of non-small cell lung cancer cells were significantly higher than that of bronchial epithelial cells (P < 0.01). The tau value of cancer cells was significantly smaller than that of bronchial epithelial cells (P <0. 0 0 1). Conclusions (1) There are potassium channels similar to delayed rectifiers in the cell membrane of non-small cell lung cancer, and their expression is significantly increased and activity is significantly enhanced. (2) Detection of K+ channels in lung cancer cell membranes can provide a theoretical basis and experimental basis for the study of signal transduction in the occurrence, development, invasion, and metastasis of lung cancer.