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目的探讨一氧化氮(NO)、丙二醛(MDA)等8项氧化损伤生化指标筛检煤工尘肺(CWP)的价值,为早期发现可疑患者和CWP的防治提供可能的线索。方法分别采用硝酸还原酶法、硫代巴比妥酸显色法、亚硝酸盐形成法、改良Hafenan氏法、尿酸酶-过氧化物酶偶联法、对苯二胺盐酸盐法及化学比色法测定112例CWP患者(CWP组)和144例与CWP组同煤矿的健康接尘对照者(对照组)血浆氧化损伤指标NO、MDA、铜锌超氧化物歧化酶(CuZu-SOD)、谷胱甘肽过氧化物酶(GSH-Px)、尿酸(UA)、铜蓝蛋白(CP)、诱导型一氧化氮合酶(iNOS)及总抗氧化能力(T-AOC)的水平或活力。结果 CWP组与对照组比较,除CP差异无统计学意义(P>0.05)外,其他各项指标比较差异均有统计学意义(P<0.05)。受试者工作特征(ROC)曲线下面积比较,MDA、T-AOC、NO和iNOS的曲线下面积大于基准线的面积,差异有统计学意义(P<0.05)。T-AOC、iNOS、MDA及NO作为CWP的早期筛检指标,以T-AOC最佳,其灵敏度达70.5%,特异度为68.1%。结论 T-AOC是8项CWP早期筛检指标的最佳指标。
Objective To explore the value of 8 oxidative damage biochemical indexes, such as nitric oxide (NO) and malondialdehyde (MDA), in the screening of coal worker’s pneumoconiosis (CWP), so as to provide clues for early detection of suspected patients and prevention and treatment of CWP. Methods Nitrate reductase method, thiobarbituric acid chromogenic method, nitrite formation method, modified Hafenan’s method, uricase-peroxidase coupling method, phenylenediamine hydrochloride method and chemical The levels of NO, MDA and CuZu-SOD in plasma of 112 cases of CWP (CWP group) and 144 cases of CWP group (control group) were measured by colorimetry. , GSH-Px, UA, CP, iNOS and T-AOC, or vitality. Results Compared with the control group, there were significant differences in other indexes except CWP (P <0.05) except CW in the CWP group (P <0.05). The area under the curve of ROC curve was significantly higher than that of baseline (P <0.05). The area under the curve of MDA, T-AOC, NO and iNOS was significantly higher than that of baseline. T-AOC, iNOS, MDA and NO were the best screening indicators for CWP. The best T-AOC was 70.5% and the specificity was 68.1%. Conclusion T-AOC is the best index of 8 CWP early screening indexes.