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目的:探讨脑还丹对由Aβ25-35诱导的实验大鼠Aβ25-35抗体和PC12细胞的凋亡及其炎性因子的影响。方法:将SD大鼠分为对照组、Aβ组、脑还丹高剂量组、脑还丹低剂量组,除对照组外,其余各组分别于实验第1天、第15天和第45天接种Aβ25-35,接种完毕后,中药组予相应药物治疗105d,各组分别在每次接种后第7天取血检测Aβ25-35抗体滴度。另外,培养PC12细胞,分组同上,除对照组外,各组分别加入Aβ25-35,同时中药组加入相对应含药血清,培养72h后检测bcl-x L mRNA和IL-1β、IL-6、TNF-α蛋白含量。结果:各组抗体量均高于对照组(P<0.01),脑还丹高剂量组产生抗体量高于Aβ组与脑还丹低剂量组(P<0.05);Aβ组IL-1β、IL-6、TNF-α分泌量均显著高于对照组(P<0.01),脑还丹低剂量组、Aβ组的IL-1β、IL-6、TNF-α分泌量均高于脑还丹高剂量组(P<0.01);Aβ组与脑还丹低剂量组bcl-x L mRNA的表达较对照组和脑还丹高剂量组低(P<0.01)。结论:脑还丹具有促进特异性Aβ25-35抗体产生的作用,同时其抗凋亡作用可能是通过降低IL-1β、IL-6、TNF-α炎性因子的水平实现的,并呈剂量依赖性。
Objective: To investigate the effects of Naoxu Dan on Aβ25-35-induced Aβ25-35 and PC12 cell apoptosis and inflammatory cytokines in experimental rats. Methods: The SD rats were divided into control group, Aβ group, high dose of brain Huan Dan group, low dose of Naohuan Dan group, except the control group, the other groups were respectively on the 1st, 15th and 45th day Inoculation Aβ25-35, after vaccination is completed, the Chinese medicine group to the corresponding drug treatment 105d, each group were seized on the 7th day after each test blood Aβ25-35 antibody titer. In addition, PC12 cells were cultured in the same manner as above, except for the control group, Aβ25-35 was added into each group, meanwhile, the corresponding drug-containing serum was added into the Chinese medicinal group. After 72 hours of culture, bcl-xL mRNA, IL- TNF-α protein content. Results: The antibody level in each group was higher than that in the control group (P <0.01). The antibody level in the high dose of BND group was higher than that in the Aβ group and the low dose group (P <0.05) (P <0.01). The secretion of IL-1β, IL-6 and TNF-α of low dose group and Aβ group were higher than those of control group Dose group (P <0.01). The expression of bcl-x L mRNA in Aβ group and YBD low dose group was lower than that in the control group and the high dose YBD group (P <0.01). CONCLUSION: Naohuan Dan can promote the production of specific Aβ25-35 antibody, and its anti-apoptotic effect may be achieved by decreasing the levels of IL-1β, IL-6 and TNF-α inflammatory factors in a dose-dependent manner Sex.