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Objective:To investigate the expression of miR—218 and its clinical significance in osteosarcoma tissues and explore its effect on proliferation and apoptosis in osteosarcoma cells.Methods:miR-218 expression was detected in 76 samples of surgically resected osteosarcoma and matched normal tumor-adjacent tissues using quantitative reverse transcription polymerase chain reaction(qRT-PCR).MiR-218 was over-expressed by exogenous miR-218 plasmids in Saos-2 cells,and then BrdU cell proliferation assay and flow cytometry were used to determine cell proliferation and apoptosis.Results:The expression of miR-218 in osteosarcoma tissues was significantly lower than those in normal tumor-adjacent tissues(t=8.735.P<0.001).MiR-218 expression in tumor tissues was significantly correlated with tumor size(x~2=5.380,P=0.020).clinical stage(x~2=6.692,P=0.010) and distant metastasis(x~2=4.l80.P=0.041).MiR-218 was obviously overexpressed by exogenous miR-218 plasmids(t= 19.42.P<0.001),and miR-218 overexpression significantly reduced cell proliferation(t=9.045.P<0.001) and induced apoptosis(t=12.38,P<0.001) in Saos-2 cells.Conclusions:The low-expression of miR-218 is correlated with the poor clinicopathological features in osteosarcoma.Moreover.miR-218 overexpression reduces cancer cell proliferation and induces apoplosis in Saos-2 cells,suggesting that miR-218 may play a key role in the progression of human osteosarcoma.
Objective: To investigate the expression of miR-218 and its clinical significance in osteosarcoma tissues and explore its effect on proliferation and apoptosis in osteosarcoma cells. Methods: miR-218 expression was detected in 76 samples of surgically resected osteosarcoma and matched normal tumor-adjacent tissues using quantitative reverse transcription polymerase chain reaction (qRT-PCR). MiR-218 was over-expressed by exogenous miR-218 plasmids in Saos-2 cells, and then BrdU cell proliferation assay and flow cytometry were used to determine cell proliferation and apoptosis .Results: The expression of miR-218 in osteosarcoma tissues was significantly lower than those in normal tumor-adjacent tissues (t = 8.735.P <0.001). MiR-218 expression in tumor tissues was significantly correlated with tumor size = 5.380, P = 0.020) .clinical stage (x 2 = 6.692, P = 0.010) and distant metastasis (x 2 = 4.l 80.P = 0.041). MiR-218 was obviously overexpressed by exogenous miR-218 plasmids (t = 19.42. P <0.001), and miR-218 overexp Conclusions: The low-expression of miR-218 is correlated with the poor clinicopathological features in (t = 9.045.P <0.001) and induced apoptosis suggesting that miR-218 may play a key role in the progression of human osteosarcoma. Osteosarcoma. Moreover. miR-218 overexpression reduces cancer cell proliferation and induces apoplosis in Saos-2 cells, suggesting that miR-